Controlling element components function in a tissue culture system

The S and M functions of En (as described in the previous note) have been demonstrated to be active in cultured endosperm tissue. Using controlled receptive alleles, wx-m-8, c-m(r) and a-m-2 in combination with the regulatory element, En, changes to Wx, to C and to A are triggered in cultured callus in a manner similar to the expression observed in kernels. With a-m-2, both functions of En, M and S are observed to function. The callus phenotypes are anthocyanin (for C and A) and amylose-staining starch (for Wx).

Mark B. Gorman and Peter A. Peterson


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