Four basal media were tested. These were two media (N6 and Yu Pei) developed by Chinese workers, Linsmaier and Skoog's and Potrykus' media for maize protoplasts. Amino acids and hormones were added in various combinations resulting in the testing of 145 variations of media. At least 50 anthers were tested on each kind of medium. Several maize strains were tested. Positive results as evidenced by the formation of 4 identical nuclei in pollen grains were obtained on a modified Yu Pei medium. Limited callus growth was obtained but no plants have been regenerated.
Colette Nitsch, M. G. Neuffer and William F. Sheridan
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