In our studies of PGM isozymes, we have discovered that two loci are associated with the variation found in maize. Although resolution of many of the bands can be made using starch gels with a buffer system consisting of L-histidine and citric acid (pH 6.5), resolution of some overlapping bands can be accomplished only by lowering the pH of the buffer to 5.0. Therefore, we routinely use both buffer systems in our electrophoretic analyses of PGM isozymes.
We have identified 13 alleles at the Pgm1 locus and 6 alleles at the Pgm2 locus. Analyses of data from five different F2 populations suggest that the two loci segregate independently (Table 1).
C. W. Stuber and M. M. Goodman
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