Regeneration from maize anther calli

During the last year we had encouraging developments with our maize anther culture. For the first time in many years we obtained two plantlets from regeneration of calli. Maize genotypes of King Huang 9 and King Huang 13, both from China, were employed in this experiment. Anthers were grown in both MIS and N6 (Chu's) media for six weeks and they were then transferred to regeneration medium without any 2,4-D or TIBA. Three weeks later, two regenerations appeared. However, both of them were finicky and failed to grow into mature plants. Now further experiments are being carried on with refined techniques. It is hoped to have more pollen plants developed in the near future.

Y. C. Ting and Margaret Yu


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