Since a wide range of morphological variations among callus lines was consistently found, it seems important to know how these lines differ in their protein contents. A standard polyacrylamide gel electrophoresis (PAGE) was adopted with a concentration of 7.5 to 9.5%. The sample concentration of one gram per 2 ml of buffer gave best results. For one callus line (81-E), as many as 15 bands could be discerned. However, the protein contents of these lines are generally very low, and it is necessary to have a large amount of callus tissues and a large volume of sample solutions for one experiment. The number of electrophoretic bands varied from one callus line to the other. It seems likely that by this approach it may throw some light on the mechanism of callus development and differentiation.
Margaret Yu and Li-san Gong
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