Analysis of genetic variability in regenerated inbred lines

For some strains, with which we can get the cycle of callogenesis and regeneration of fully fertile plants with normal chromosome number, we are going to study the genetic variability, after long callogenesis, within and between the progenies of the regenerated plants in comparison with normal selfed progenies from the original genotype. Biometrical traits were studied after two sexual reproduction generations to try to remove the effect connected with the seed quality or at a hybrid level in order to decrease, in an agronomic trial, the importance of environmental variation. The general protocol is described in Figure 1. The following traits were measured: number of leaves for some given developmental stage; plant and ear heights; leaf area; flowering earliness; grain yield components.

In a first experiment we compared with the check 23 regenerated plants from the line CO158 which were crossed with the line F1444 as male parent. The analysis of traits showed a varietal effect as far as the means are concerned and the comparison of the variances (within varieties) revealed heterogeneity between them. A global comparison for all traits for all varieties showed the following:


Two regenerated plants seem to have an unusual behavior.

We are studying now two other groups of regenerated plants with the same general protocol for the inbreds A641 and A188.

Figure 1.

Michel Beckert, Maurice Pollacsek and Ming Quing Cao

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