45 EMS-induced virescent mutants that have been located to 11 chromosome arms by the B-A translocation method were chosen to test for allelism. The objective is to determine the number of duplicate events in the target material and to identify new virescent loci. Virescent mutants were chosen because they are viable, allowing mutant by mutant crosses. If both homozygotes are not obtained then crosses of known heterozygotes or, in the worst cases, 2/3-right (unknown) heterozygotes from a segregating M2 must be used. In the latter case, several crosses are needed for each valid test. Fifty seeds from each test ear were planted in a sandbench and screened. The results showed that some mutants are alleles.
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