Protoplast culture in maize

Three cell suspension cultures have been obtained from three independent isolations of friable callus cultures originating from immature embryos of WF9 inbred line carrying the S-cms trait. Protoplasts are readily obtained from these cell lines under conditions described previously (TAG 59:341). These protoplasts would regenerate cell wall as visualized by the appearance of calcoflour fluorescence after about 48 hours of culture. However, no sustained cell divisions have been seen so far in these cells under conditions in which protoplasts derived from Black Mexican cell line divide and proliferate to give cell colonies.

Prem S. Chourey

Please Note: Notes submitted to the Maize Genetics Cooperation Newsletter may be cited only with consent of the authors.

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