Localization of Got2 isozymes in maize

In our studies of enzymes associated with plastids (etioplasts), we have found isozymes encoded by Got2 in this plastid fraction. An earlier report (Scandalios et al., 1975, Biochem. Genet. 13:759-769) indicated that isozymes encoded by Got2 were found exclusively in the cytosol, however the plastid fraction may have been included with the cytosol in their analyses. In our studies, activity of Got2 isozymes in seedling leaves is considerably greater than that found in coleoptiles. This would be expected for isozymes localized in the plastids.

C. W. Stuber and M. M. Goodman


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