Earlier we have reported (MNL, 1985) the effect of et mutation
on chlorophyll pigmentation and chloroplast ultrastructure during greening
of seedlings under light. We report here on the changes in total amylase
in germinating kernels and young seedlings. The total amylase levels were
estimated in et+ et+ and et et kernels during germination
and greening of seedlings (Table). The total amylase levels and the specific
activity of amylase were observed to increase from the 3rd day to the 5th
day after germination in et+ et+ kernels, whereas in et
et kernels the activity levels decreased during the same period.
Further, the total amylase content was significantly higher in et+et+
kernels as compared to et et kernels at all tested stages.
In the developing seedlings too, the total amylase levels were significantly
higher in normal (et+ et+) leaves than that of virescent
leaves of et et genotype. Total amylase levels peaked at
the 8th day followed by a decrease by the 10th day in leaves of both genotypes.
Similarly, the specific activity of total amylase peaks at the 8th day
in et+et+ seedlings. However, the mutant seedlings show continuous
increase up to the 10th day. a
amylase studies in kernels and seedlings of both genotypes
are in progress. Electrophoretic profiles of total amylase did not show
any qualitative differences between et+ et+ and et
et genotypes throughout the period of kernel germination.
|Days after germination||Total amylase activity* (n.Kat/mg kernel/gm.leaf)|
|et+ et+||et et|
*Each value represents an average of at least 5 independent experiments.
H.G. Sangeetha and A.R. Reddy
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