RFLP mapping of cloned genes and their related sequences

One of the many applications of restriction fragment length polymorphisms (RFLP) in plant genetics is determination of the chromosomal location of cloned gene sequences. In last year's news letter (Wright et al., MNL 61:89) we reported the mapping of 21 such cloned genes or related sequences in maize. Since that time, we have received and utilized another 9 genes in our RFLP studies, some of which had their genomic origins determined previously through conventional linkage analysis. As most of them hybridized to numerous locations in the genome, this resulted in the addition of 22 loci to our RFLP linkage map, bringing the total number of loci on our current map to 370 markers (Helentjaris et al., Genetics, in press). The following is in addition to this previous list of cloned genes, the approximate chromosomal location of them or related sequences, and the individuals who provided them.

Anyone who is currently generating clones of known identity and would be interested in having their genomic location investigated, please feel free to contact us. If you are interested in using any of the above cited clones for your own research, please contact the original providers as we do not distribute these clones, only those generated in our own programs.

Table.

Scott Wright and Tim Helentjaris


Please Note: Notes submitted to the Maize Genetics Cooperation Newsletter may be cited only with consent of the authors.

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