Effect of gibberellic acid on growth and regeneration of callus

During last fall, our callus lines SAN1, SDN1 and 81-B5B were treated with varying amounts of gibberellic acid (GA) ranging from 0.1 to 5.0mg/l. The GA was mixed with regeneration media having 2% sucrose. Each treatment had at least 5 replicates and 3 controls. It was found that cultures of SAN1 and SDN1 containing 1 to 2 mg/l GA produced more prolific growth with higher chloroplast content than those with the other treatments. In addition, these cultures also had some regenerations. A few of the calli had 2 to 4 somatic embryos. These embryos, however, did not continue development and differentiation. Most of them stopped at the leaf primordium stage. For callus line 81-B5B, no positive responses were observed.

Y.C. Ting and Stephen Schneider


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