Micro-propagation through axillary bud culture: an update with four different genotypes --V. R. Bommineni, E. Banasikowska, and D. B. Walden We have reported a summary of our axillary bud culture data (cv. Seneca-60) (Walden et al., Maydica 34: 263-275, 1989) derived from twelve original explant lines for up to 13 transfer generations. One of the original 12 explants has been maintained for 21 transfer generations (Table 1). We began recently to extend this system to important inbred lines and genetic stocks as extensive micro-propagation of axillary buds permits clonal propagation of maize.

The original explant of each genotype was derived from a glasshouse grown, 20-30 day old seedling. The stalk of the seedling was surface sterilized in 20% 'Javex' for 20-30 min and rinsed three times with sterile distilled water. The sheaths of stalk were removed under the microscope in the culture hood to expose the shoot apical meristem. Each explant consisted of a shoot apical meristem with 5-6 nodes at the time of culture. The explant was placed in a small jar containing MS medium and other plant growth hormones (optimal) as reported in Table 1. Other incubator and growth conditions were same as in our earlier report (Bommineni et al., MNL 63: 87-88).

Table 1: Micro-propagation of maize through axillary bud culture from a single explant.


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