The separations by column chromatography (DEAE-cellulose) of the extracts from N22 and Oh43 reveal only a single peak of activity, indicating that the single zone of activity observed on SGE is unlikely to result from two isozymes that migrate to exactly the same zone.
The lack of the cytosolic isozyme has
no discernible phenotypic consequence. It appears that this enzyme is completely
dispensable for normal endosperm development. It is also possible that
the absence of this enzyme is one of the cryptic deficiencies (along with
many others) that contribute to the lessened vigor and lower productivity
that characterize inbred lines and that is often redressed by crossing
to an unrelated inbred line. This hypothesis is testable, and we are proceeding
to do so.
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