RALEIGH, NORTH CAROLINA
North Carolina State Univ. & USDA/ARS
NEW YORK CITY, NEW YORK
Lehman College, CUNY
Isolation and mapping of a cDNA probe
for the b-70 protein
--Brian B. Shank, Hank W. Bass, Paul
H. Sisco, Eleanora Wurtzel, and Rebecca S. Boston
An increase in the accumulation rate of
the b-70 protein of maize endosperm has been correlated with the zein regulatory
mutation floury-2 (Galante et al., Mol. Gen. Genet. 192:316-21, 1983).
A cDNA clone encoding b-70 was isolated from a lambda gt11 cDNA library
of endosperm mRNA (14DAP). This expression library yielded one clone encoding
a protein that cross-reacted with b-70 antiserum and corresponded to an
mRNA that increased in the fl2 mutant.
We used this cDNA clone to probe genomic
Southern blots from Ben Burr's recombinant inbred lines (Burr et al., Genetics
118:519-26, 1988). At low stringencies (Tm-35 C) several bands cross-hybridized
with the probe, suggesting that the gene was a member of a multigene family.
At higher stringencies (Tm-20 C) the probe mapped to a single locus near
BNL1.380 on Chromosome 5.
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