Effectiveness of two restriction enzymes for detecting RFLPs in the Iowa Stiff Stalk Synthetic (BSSS) maize population --E.A. Lee, M. Lee, K.R. Lamkey and W.L. Woodman BSSS progenitor and derived inbred lines were examined at 58 RFLP loci with single digests of EcoRI and HindIII. In all groups of inbreds, a higher percentage of clones detected polymorphisms when tested with HindIII digests (Table 1). Also, the average size of the restriction fragments detected with HindIII seems to be lower molecular weight than those observed with EcoRI digests, suggesting a higher frequency of HindIII recognition sequences in BSSS germplasm.

Table 1. Percentage of polymorphic clones summed over inbred groups and restriction enzymes.
 
Inbred
Restriction Enzyme
Group
EcoRI
HindIII
Progenitors
90.4%
92.7%
Elites
75.0
89.1
Random
74.5
89.1


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