--C. M. Wilson
Zeins of individual seeds from two sets of maize recombinant inbreds derived from F2 populations T232xCM37 (48 lines) and CO159xTx303 (41 lines) (Burr et al., Genetics 118:519, 1988) were assayed by IEF in agarose (Wilson et al., Theor. Appl. Genet. 77:217, 1989). Each band of the parental inbreds was given an IEF number by relating it to the positions of zeins in standard inbreds. The occurrence of zeins in three seeds from each of the recombinant inbreds was determined. Only 3 or 4 inbred lines appeared to be segregating. Loci positions were kindly assigned by B. Burr (see MNL64, 1990 maps).. Eleven zein genes were placed on chromosome 4S, four on chromosome 4L (near the centromere), and 3 on chromosome 7S (Table 1). The recombinant inbred linkages agreed with the positions suggested by the earlier work.
IEF bands previously reported (Wilson, TAG, plus MNL report above) are associated with the linkage groups as follows:ZpL1a-f (4S): 17.5, 14/19, 32, 35, 36, 37, 60;
Table 1. Linkage groups for zein genes in recombinant inbreds.
Genes for zein bands 32 and 33.5 occurred on both arms of chromosome
4. Totals for identifiably different (by IEF position alone) zeins which
have been mapped are 13 for ZpL1a/f, 11 for ZpL2a/3a, and 6 for ZpL2b,
for a total of 30 genes for AB zeins in the four recombinant inbreds plus
the 13 crosses studied earlier. Recent work (C. M. Wilson, Plant Physiol.
1991, in press) suggests that these are minimum numbers, for serial analysis
by HPLC in addition to IEF shows that apparently identical zein IEF bands
in two inbreds may differ by HPLC.
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