Database for loci mapped in TxCM and COxTx RI families

--B. Burr, F.A. Burr and E.C. Matz

Ed Coe has asked us to reproduce our laboratory database that describes everything we know about the loci mapped in the recombinant inbred families. Because this database was never intended for publication, browsers will find it both idiosyncratic and cryptic. The fields are:

1) the name of the locus

2) estimated map position

LOCUS POS. INBRED LINE ENZYME & MORPH SIZE   REMARKS
    CM37 T232 Tx303 CO159  
             
a1 3L149 Bgl2 ~9, Hin3 11 Bgl2 ~16, Hin3 7.3 Bgl2 ~6 Bgl2 ~16 probe is pAMu2 from Z. Schwarz-Sommer. 600 bp.
abp1 3L068         Auxin binding protein;data from Marian Lobler,probe = G900,saw SstI polymorphism. 10/18/90 combined with pKlamb+ of A Jones
aco1 4S059     1 4 isozyme
acp1 9L070 2 4 4 3 isozyme
acp4 1L177 5 4 4 5 isozyme
act1 8L025 Bgl2 6.0 3.9     probe is pMac1 from R. Meagher
adh1 1L128 Bam 12 Bam 16, 7.1 4 6 probe is pH2.3 from M. Freeling, isozyme in 2nd family
adh2 4S046 Hin3 11kb 5.4 Eco 7.2, Bam ~6 Eco 13.5 Bam ~5.5 probe is pZML841 from M. Sachs
agp1 6L109     BamHI 18.2 kb 8.3 kb 2.2 kb EcoRI cDNA clone.Embryo specific counterpart to sh2 from M.J. Giroux
agp2 2L106 EcoRI~24.5 kb 8.5 kb EcoRI 13.5 kb 8.9 kb 1.8 kb EcoRI cDNA.Embryo specific counterpart to bt2.From M.J. Giroux.
agr-c94 4S021 Bam 1.5 Bam 5.8 Bam 1.5 Bam 8.3 cDNA probe from Agrigenetics; 0.83 kb fragment from a Pst digest, also a .35kb.
agr-r115 4S005 Bgl2 6.8 7.8 Bgl2 5.4 10.5 Blots probed by Yu Ma with a cDNA probe.
als1 4L087     Hin3 16-17 Hin3 2.5 acetolactate pyruvate-lyase from tobacco probed by N.S. Shepherd, 1st band
als2 5L049     Hin3 9.5 Hin3 3 2nd band probed with tobacco als by n.S. Shepherd
amp3 5S038 4 3 4 3 isozyme
ars1 2S051 BglII 8.0 kb (18.2 + 16.3 kb) 19.6 kb (10.6 kb) BglII 8.0 kb (18 + 4.4 kb) 8.4 kb (5.45 + 4.7 kb) 800 bp PstI from Paul Sisco; Eileen thinks 680 bp.Should map close to b1. 10/16/90
b1 2S049 + - - - morphological
bnl1.297 3L126 Bam ~4.5 ~5.5 Bam ~10 ~15,7 same as 1.80, O.95 Eco cDNA
bnl1.326 1S016     Eco ~6.5 ~7.5 0.54 Eco cDNA
bnl1.380 5S028 Eco ~4 ~3.5 Eco ~4 ~20 0.32 Eco cDNA
bnl1.45 8L015 Eco ~6.5 ~5.5 Eco ~6.5 ~5.5 0.44 Eco cDNA
bnl1.556 1S052 Eco ~13 ~16 Eco ~18 ~15 LINKED WITH 5.59, 0.44 Eco cDNA
bnl1.80 3L126 Bam ~4.5 ~6 Bam ~12 ~16 same as 1.297
bnl2.369 8L032 Eco >25 ~14 Bgl2 ~14 6.6 0.70 kb Eco cDNA
bnl3.02 10S-07 Bam ~16 ~6.5 Bam ~24 ~10 Same as 3.04
bnl3.03 6L067 Bam 15 9 Bgl1 >25, 15.5 Bgl1 13, 4.4 2.5kb PstI genomic
bnl3.04 10S-07 Bam 22, 6.6 ~10, 6 Bam 22, 6.6 ~10. 6 LINKED WITH 5.40 AND 10.17 IN FIRST FAMILY; 2.2 kb Pst Genomic
bnl3.06 9S053 Bam ~5 ~9, 12     2.35 kb Pst Genomic
bnl3.18 3L120     Bgl2 ~6 ~8 2.1 kb Pst Genomic
bnl4.24 7L054 Bam ~6.8 ~8 Hinc2 6.1 Hinc2 6.4 2.35 kb Pst genomic
bnl4.36 5S035 Bam ~17,15 ~20 Bam ~16 ~14 2.3 kb Pst genomic
bnl5.02 5S013 Eco ~24 ~8 Eco ~10 ~8 2.2 kb Pst genomic, NPI finds second locus on 1L near centromere.
bnl5.04 9L074 Bam ~18,7 ~3 Bam ~7,(12) ~13,(9) 2.1 kb Pst genomic
bnl5.09 9L106 Bgl2 ~6.6 ~16 Eco ~2 ~18 2.3 kb Pst genomic
bnl5.10 9L064     Bgl2 ~5 ~16 2.4 kb Pst genomic
bnl5.14 3L097     Eco 18 Eco 5.0 2.5 kb Pst Genomic
bnl5.21A 7L065 Bgl2 5.4 Bgl2 11.5 Bgl2 6.2 Bgl2 11.5 2.2 kb Pst Genomic, 1st band probed with 5.21
bnl5.21B 2L139 Bgl2 9.0 Bgl2 7.8 Bgl2 8.7 Bgl2 8.2 2.2 kb Pst genomic, 2nd band probed with 5.21
bnl5.24 5L112 Bam ~12 ~20 Bam ~18,7 ~15,9 2.5 kb Pst genomic; B-A possibly 5L
bnl5.27 5S018     Eco 9.0 Eco 7.5 2.0 kb Pst genomic
bnl5.33 3L123 Bgl2 6.6 Bgl2 5.7 Bgl2 5.4 Bgl2 5.8, 3.0 2.1 kb Pst genomic
bnl5.37 3L084 Bam ~9 6.6 Bam ~18 and 6.6 ~12 2.3 kb Pst genomic
bnl5.40 5L067 Bam ~4.5 ~5.5 Bgl2 >25 ~22 LINKED WITH 10.12L; 2.25 kb Pst genomic
bnl5.46 4S054     Bam ~18 ~8.5 2.25 kb Pst genomic
bnl5.47 6L088 Bgl1 1.7 Bgl1 4.0 Bam ~18, 4.8 ~8, 6 2.25 kb Pst genomic
bnl5.59 1L062 Bam ~4.2 ~4.0, 1.0 Bam ~4.0 ~4.2 LINKED WITH 1.556; 2.25 kb Pst genomic; B-A not 1L, 1S, 4L, 7L, 9L, 6L false +
bnl5.61A 7L066 Bgl2 6.6, 5.5 Bgl2 12 Bgl2 6.4 Bgl2 11.5 2.2 kb Pst genomic, 1st band probed with 5.61
bnl5.61B 2L143 Bgl2 9.4 Bgl2 7.7 Bgl2 8.9 Bgl2 8.2 2.2 kb Pst genomic, 2nd band probed with 5.61
bnl5.62 1S-31 Bam ~9. 6.8 ~10 Bam ~24, 10 ~9.5, 7 2.1 kb Pst genomic; NPI finds a second locus on 2S between lg and b.
bnl5.67 4L141 Bgl2 ~1.8 ~2.6 Bgl21.8 ~2.6 LINKED WITH SSU. 10.05, 7.65; 2.5 kb Pst genomic; B-A positive for 4L, not 9L
bnl5.71 5L053 Bgl2 ~20 ~5 Bam ~18 ~4.5, 5.5 2.5 kb Pst genomic
bnl6.06 3L069 Bam ~6.5 ~9.5,4.5 Bam ~9.5,4.5 ~3 2.4 kb Pst genomic
bnl6.10 5S015     Eco ~25 ~15 2.1 kb Pst genomic
bnl6.16 3L115 Bam ~5.5,(6.9) ~6.8 Bam ~6.8 ~8,(6.6) 2.45 kb Pst genomic
bnl6.20 2L151 Bam ~6 ~10 Bam ~9, 4.5 ~10 Tightly linked w/ NPI298; 2.5 kb Pst genomic
bnl6.22 5S026 Eco ~20 ~7     2.0 kb Pst genomic
bnl6.25 5S-25 Bam ~4.4 ~4.8 Bam ~4.5 ~12 linked with 8.33; 2.25 kb Pst Genomic; B-A not 1L, 3L, 7L, 9L, 10S, possibly 2L
bnl6.27 7L056 Bgl1 20, >25 Bgl1 18.5 Bgl1 17.5 Bgl1 >25 2.2 kb Pst genomic
bnl6.29 6S001 Eco 21 14     2.3kb PstI genomic; this clone originally called 6.24
bnl6.32 1L172 Bam two lower ~18 Bam 4.4 18 2.25 kb Pst genomic
bnl7.08 8L021 Bgl2 4.5 25 Bgl2 25 4.5 2.3kb PstI genomic; upon regrowing this plasmid found a second 2.4kb insert (?)
bnl7.13 9L075 Bgl2 ~5.5 ~5.0 Bgl2 ~7 ~5 2.1 kb Pst genomic
bnl7.20L 4L078 Eco 14 3.2, 2.3 Eco 4.1, 21.5 9.2, 3.1 2.4 kb Pst genomic, a higher Pst band in this clone was not a good probe
bnl7.21 1S042 Bam ~7 ~2.4     2.5 kb Pst genomic
bnl7.24A 9L061     Bam 3.2 Bam 7.8 2.1 kb Pst genomic, 1st band probed with 7.24
bnl7.24B 5S-16     Bam 2.3 Bam ? 2.1 kb Pst genomic, 2nd band probed with 7.24
bnl7.25 1L138 Eco ~13 ~7 Eco ~7.5 ~13 2.5 kb Pst genomic
bnl7.26 3L165     Eco 17 20 2.4 kb Pst genomic
bnl7.28 6L009 Bam 20 8.9, 3.3 Bam 8.8 11.7 2.3 kb Pst genomic
bnl7.43 5S032 Bam 6.7 ~18 Eco ~18 ~6 2.4 kb Pst genomic
bnl7.49 10L080 Bam ~20 ~16 Bam ~21(doublet) ~18 TIGHTLY LINKED WITH 17.02; 2.1 kb Pst genomic; B-A not 4L, not 6L
bnl7.50 9L091 Bam 22 3.6 Bam 5.8 22 2.3 kb Pst genomic
bnl7.56 5S010 Bam ~25 ~9.5 Bam ~9.5 ~25 2.2 kb Pst genomic
bnl7.57 9L110 Bam 7.7 Bam 6.0 Bam 7.1 Bam 6.5 2.5 kb Pst genomic
bnl7.61 7L077 Bam ~6.5 ~8.5 Bam ~20 ~9 1.8 kb Pst genomic
bnl7.65 4L106 Bam ~7 ~12 Bam ~15 ~8 LINKED WITH SSU, 10.05, 5.67; 2.2 kb Pst genomic; B-A probably 4L
bnl7.71 5S039 Bam >30 22 Bam ~15 ~20 2.55 kb Pst genomic; B-A not 2L, 3L, 9L
bnl8.01 3L095 Bam ~20 ~5 Bam ~6 ~12 2.4 kb Pst genomic
bnl8.04 2S070     Bgl2 ~23 ~25,20 TIGHTLY LINKED WITH 12.36; 2.5 kb Pst genomic
bnl8.05 1S-28 Bgl2 ~7.2,7.0 ~6.8 Bgl2 ~6.8,3.0 ~7.2,6.6 2.3 kb Pst genomic; linked w/ 5.62; B-A 1S confirmed, not 4S, 7L, 8L, 10L
bnl8.06A 8L017 EcoRI4.7 kb 13.0 kb EcoRI 14.0 kb 18.5 kb 2.25 kb Pst I genomic
bnl8.06B 6L092 EcoRI 11.5 kb 2.9 kb EcoRI   Minor band probed with 8.06; polymorphism in first family only.
bnl8.08 1L146 BamHI 2.7 kb 3.1 kb     2.15 kb Pst I genomic.Minor band.2F doesn't show parental polymorphism.
bnl8.10 1L112 Bgl1 2.5 Bgl1 7.7 Eco ~18 ~15 2.5 kb Pst genomic
bnl8.15 3S010 Bgl ~5.5 ~5 Hinc2 18.5 Hinc2 10.0 LINKED WITH E8; 2.1 kb Pst genomic
bnl8.17 9L086 Bam ~14 ~20 Bam ~7 ~20 2.2 kb Pst genomic
bnl8.21 7L079 Bam 10 23 Bam (20), 8 5 2.2 kb Pst genomic
bnl8.23 4L156 Eco 7.4 7.9 Eco 9.0 4.5 2.5kb PstI genomic
bnl8.26 8L037     Eco ~10 ~9,5.5 2.4 kb Pst genomic
bnl8.29A 1L152 Hin3 11 Hin3 14 Eco ~4, Hin3 16 Eco ~9, Hin3 12.5 2.3 kb Pst genomic
bnl8.29B 5S-20 Hin3 6.5, 3.3 Hin3 8.0 Hin3 12 Hin3 3.0 2.3 kb genomic, 2nd band probed with 8.29
bnl8.32 7L070 Bam 10 2.3 Bam 10 2.4 2.4 kb Pst genomic
bnl8.33 5S-38 Bam 4.4 ~5.5, 4.0 Bam ~4.8 4.3 linked with 6.25; 2.5kb PstI genomic
bnl8.35 3S054 Bgl2 23 4.5 Hinc2 18 Hinc2 2.0 2.2 kb Pst genomic
bnl8.37 7L078 Bgl2 ~25 ~18 Bgl2 ~20 ~15 2.1 kb Pst genomic
bnl8.39 7L087 Hin3 11.6 Hin3 22 Bam 4.4, 2.5 ~5 2.2kb PstI genomic
bnl8.44 7L122 Bam 6.7 ~15, 8 Bam ~10 ~15, 7 TIGHTLY LINKED WITH 16.06; 2.2 kb Pst genomic
bnl8.45 2S010 Bam ~7.5 ~6 Bam ~7 ~7.5 linked with NPI 239; 2.1 kb Pst genomic
bnl9.07 9S006     BamHI 17.2 kb 21.5 kb 2.4 kb PstI genomic
bnl9.08 8L017 Bam ~15,8 ~7,6 Bam ~18,9 ~25,17 2.2 kb Pst genomic. Hybridize at 50c.
bnl9.11 8S-19 Bam 22 10 Eco ~25 ~20,16 linked with 15.07, 13.05; 2.4 kb Pst genomic; B-A not 4L
bnl9.13 1S-01 BglII 7.1 2.1     2.0 kb PstI genomic
bnl9.44L 8L012 Bgl2 ~10 ~12 Bgl2 ~12, 9 ~10 0.5 kb Pst genomic
bnl10.05 4L104 Eco ~15, 7 ~12 Eco ~18 ~16 LINKED WITH SSU, 7.65, 5.67; 2.5 Pst genomic; B-A probably 4L, not 3L, not 10S
bnl10.06 5S030 Eco ~6 ~18 Eco ~18 ~6. (18) 2.3 Pst genomic
bnl10.12L 5L058 Eco ~9.5 ~15 Bgl2 ~10 ~4.8 2.2 kb PstI genomic
bnl10.12U 8L087 Bam ~4.6 ~9.2 Bgl2 ~9 ~13 2.5kb PstI genomic; TIGHTLY LINKED WITH 10.24 IN SECOND FAMILY; B-A not 4L
bnl10.13 10L059 Bam 2.9 4.4 Eco 9.2 22 2.2kb PstI genomic
bnl10.17 10S-17 Eco ~8.5 ~9 Bcl 5.9 5.6 LINKED WITH 3.02 AND 3.04; 2.6 Pst genomic
bnl10.24A 3L089 Bgl2 ~8.5, Bam >25 Bgl2 ~5.5, Bam 19 Bam 23 Bam >25 29 Jan `87, poor; 2.2 Pst genomic (same as 10.24B)
bnl10.24B 8L081 Bam 5.1, 4.5 Bam 6.3 Eco ~3, Bam 6.8 Eco~2.9, 1.0, BAm 4.6 9 Dec `86; TIGHTLY LINKED WITH 10.12U IN 2ND; 2.2 Pst genomic (same as 10.24A)
bnl10.38 1S014     Eco ~5,3.5 ~18 2.3kb PstI genomic; linked with 1.326
bnl10.39 8L008 Eco ~25 ~16     2.0 Pst Genomic
bnl10.42 2S069 Bgl2 ~3 ~6(doublet)     tightly linked with 12.36; 2.6 Pst genomic
bnl12.06 1S031 Bam lower upper Bam >25, 20 24, 19 2.0 Pst genomic
bnl12.09 2L082 Bam 4.1, 1.5 2.0     1.3kb Pst genomic
bnl12.30A 8L048 Eco 9.9 15.3 Eco 15.3 6.3 1.6kb PstI genomic
bnl12.30B 3L150 EcoRI       Data from Paul Sisco. 12/14/90
bnl12.36 2S066 Eco ~13 ~20 Eco ~4 ~7 LINKED WITH 10.42 AND 8.44; 2.1 Pst genomic
bnl13.05A 8S-34 BglII 2.95 kb + 950 bp 1.33 kb (19.7 kb) Bam 22.5 kb 17.5 kb 1.3kb PstI genomic; A second locus on 3L; B-A not 4S, 8L, 10L; 1F redone 10/16/90
bnl13.05B 3S063 BglII 13.5 kb 17.5 kb Eco 23 kb 7.8 kb 2nd band probed with 13.05. 2nd family data from 20 Jan blot(Ben's); 1F redone 10/16/90
bnl13.05C 8L013 BglII 21.5 kb 22.5 kb     10/17/90
bnl13.24 7L059 Bam ~10 ~20 Bam ~20 ~18 1.45kb Pst Genomic
bnl14.07 7L076     Eco ~20 ~6.8 1.2kb Pst genomic
bnl14.26 9L104 Bam ~15 12 Bam 9.5 ~16 same as 14.28
bnl14.28 9L104 Bam ~14 ~10 Bam 7 ~15 1.55kb Pst genomic; B-A on 9L, not on 2L, 4L, 10S
bnl14.34 7L076     Eco ~20 ~6.6 same as 14.07
bnl15.07 4L163 Bam 6.5 4.8 Bam 4.5, 2.4 6.8 Linked w/ 8.23;Loosely linked with 9.11, 13.03; B-A not 2L;1.95kb Pst genomic; 1/14/91
bnl15.18 1L123 Bam 9.5 3 Bam 7.5 2.8 1.4 kb Pst genomic
bnl15.20 3L112 Eco ~13 ~8 Bam ~9 ~7, 5 1.1 kb Pst genomic
bnl15.21 7L046 Bam ~22, (16) ~18, 12 Bam ~25, 30 ~15 1.25 kb Pst genomic; B-A possibly 7L
bnl15.27 4L073 Bam 9.3 11.5     1.3kb Pst genomic; tightly linked with 15.45; B-A not 7L
bnl15.37 6L078 Bam ~25, (20) ~14 Bam ~18, 15, 12 ~22 1.15 kb Pst genomic
bnl15.40 7S028 Bam ~10, 7, 5.5 ~15 Bam ~6 ~6.5 1.95 kb Pst Genomic
bnl15.45 4L073 Bam 15.5, 2.7 15.5. 14 Bam ~13, 8 ~2.8 1.3 kb Pst genomic; B-A not uncovered by 4L
bnl16.06 7L114 Bam ~22 ~9 Bam ~17 9.5 LINKED WITH 8.44; 1.8 kb Pst genomic; B-A positive for 6L
bnl17.01 8L055 Hin3 3.0 Hin3 2.2 Bgl2 15 8.6 This is a second band probed with the A1 clone pAMu2
bnl17.02 10L065 Bgl2 ~6 ~1.8 Bgl2 ~4 ~1.8 random genomic from B. Lowe and P. Chomet, 1.2 kb Eco
bnl17.03 2L166 Eco 9.3 5.3 Eco 3.3, Hin3 5.1 Eco 5.1, Hin3 5.7 This is a second band detected with the C2 cDNA probe; it may be Whp1
bnl17.04 1L124 Eco 8, 4 7 Eco 8, 7 3, 2,5 pUC9 -1 alpha tubulin probe from D.P. Weeks
bnl17.05 4L115 Bam 2.1 ~1.8 Bam ~1.8 2.1 LINKED WITH 10.05, 7.65, 5.67; SSU probe is pC1 from R. Broglie
bnl17.06 1L099 Bam ~9 ~12.5 Bam ~20, 15 ~9 probe is p7.6-1, random genomic from K.C. Cone, 2 kb Acc, subcloned with Sal end
bnl17.07 10L060 Bam 8.4 4.2 Bam 4.2 12.4 probe is 1.85kb subgenomic in pBF245; B-A 10L confirmed
bnl17.09 4S069     Hinc2 9.0 Hinc2 5.8 This was called umc59, but is an unknown probe.
bnl17.12 6L095 EcoRI 4.9 kb 5.7 kb EcoRI 5.6 kb 4.8 kb cDNA subclone pBF263B (9.1.1L), 0.7 kb Eco insert.
bnl17.13A 7S022     BglII 20 kb + 11 kb ~25 kb + 9.9 kb + 7.2 kb 550 bp EcoRI insert. cDNA subclone pBF263F. Lambda clone 11.2.1AL. 10/16/90
bnl17.13B 4S035     BglII 5.8 kb 8.8 kb + 5.3 kb 550 bp EcoRI insert. cDNA subclone pBF263F. Lambda clone 11.2.1AL. 10/16/90
bnl17.13C 4S063     BglII no band 7.6 kb 550 bp EcoRI insert. cDNA subclone pBF263F. Lambda clone 11.2.1A. 10/16/90
bnl17.14 2L189 BglII 6.9 kb 6.0 kb BamHI 18.2 kb 5.9 kb 1.25 kb PstI, Received as pio200075, but detected no linkage with 10S.
bnl17.15 1L082 BglII 5.4 kb 6.7 kb BglII 6.8 kb 11.5 kb 1.65 kb Eco. Detected w/ bt2 cDNA from Hannah = adp1? 12/14/90
bnl17.16 8L019 BglII 2.0 kb No band     Minor band probed by bt2. May be same as adp4. 1/22/91
bt1 5S042 BglII 9.8 kb 6.8 & 4.85 kb BglII 10 kb 23.1 kb 1.85 kb EcoRI insert from cDNA clone from Tom Sullivan. 12/14/90
bt2 4S067     BglII 8.2 kb 5.6 kb 1.65 kb EcoRI. cDNA clone from Curt Hannah. 12/14/90
bz1 9S031 Eco ~5 Eco ~21 Eco Eco probe is pMBzPA from D. Furtek. Hybridize at 50c.
bz2 1L106 Bgl2 8.6 Bgl2 7.5 Bgl2 7.5 Bgl2 8.6 probe is pP300 from V. Walbot
c1 9S026 Bam 9.9 Bam 8.8     probe pEco1.0 from K.C. Cone
c2 4L117 Eco 10.2 15 Hin3 20 Hin3 9.2 Probe is 1.5kb maize cDNA clone from U. Wienand
dia1 2L109 8 12     isozyme
e8 3S020 4.5 4 4 5 isozyme
enp1 6L016     6 10 isozyme
gln1 10L095 Bam 15.5 9.6 Bam9.4 4.8 Probe is glutamine synthetase clone GS6.15:PBS+, 1350bp Eco insert from Messing.
glu1 10S026 7 2 7 6 isozyme
got2 5L096 2 4     isozyme
hcf106 2L092         Data from Rob Martienssen
hex2 6L062 1 2 2 1 isozyme
idh1 8L063     6 4 isozyme
idh2 6L105 6 4 6 4 isozyme
mah9 5S004 EcoRI 8.9 kb 5.9 kb EcoRI 4.8 kb + 4.4 kb 6.0 kb 300 bp Pst cDNA frag. from M. Pages; 10/16/90
mdh1 8L002 6 1     isozyme
mdh2 6L107 3.5 6 3 6 isozyme
mdh3 3L146 16 18 18 16 isozyme
mdh4 1L097     14.5 12 isozyme
mdh5 5S017 N 15     isozyme
me2 6L076         NADP-dependent malic enzyme, cytosolic; data of Beverly Rothermel (Yale).
mgs1 10L032 BamHI 5.6 kb 5.4 kb BamHI 5.0 kb 5.5 kb 450 bp EcoRI + HindIII; this is zmc13 from Mascarenhas; Pl.Cell 1:173(1989).
mgs2 4L127 EcoRI 5.95 kb 8.0kb EcoRI 5.95 kb 3.75 kb Pectinalyase cDNA clone zmc58 from Mascarenhas.Pollen specific.
nabp1 7S026     Hin3 ~7.6kb 8.8 Data of Bill Cook, Missouri; nucleic acid binding protein, on top of pio200690B
ncr-nrA 1L076 HindIII   EcoRI   Data from Paul Sisco. 12/14/90
ncr-nrB 4L074 HindIII   EcoRI   Data from Paul Sisco. 12/14/90
ncrb32c3A 8L023         Data from Paul Sisco.B32 endosperm protein family probe from B. Boston.
ncrb32c3B 7L083         Data from Paul Sisco.B32 endosperm protein family probe from B. Boston.
ncrb70 5S031         Data from Paul Sisco.Probe from B. Boston.
niu1 8L010 Eco 2.3 2.7 Eco 3.05 2.25 Probe was 550 bp SalI-SphI fragment from Bs1; data from Rick Johns.
niu2 5S008 Eco 2.3 2.7 Eco 3.05 2.25 Probe is 550 bp Sal-Sph fragment from Bs1; data from Rick Johns.
nor 6S-06 Sst 6.6 Sst 11.5 Sst 4.2 Sst- probe is pBF243
npi97 1S-13 Bgl2 12.3 Bgl22.0 Bcl1 3.0 Bcl1 8.0 1.1 kb HindIII fragment
npi98 6L036 Eco 2.9 Eco 2.3 Eco 2.85 Eco 2.2 1.2 kb insert cut out w/ H3; npi98 is minor band & npi225 is major band w/ probe. 1/14/91
npi105 10S027 EcoRI 9.1 kb 7.6 kb BglII 12.2 kb 13.4 kb 780 bp PstI.This is most proximal probe on 10S.
npi109 1S-04          
npi113 7L111 Bgl2 19.5 12.5 Bgl2 22 19, 8 1.4 kb Hind III insert.
npi114 8S-55 HindIII   EcoRV   Data from Paul Sisco. 12/14/90
npi118 2L149 Bgl2 7.7 17 Bgl2 12 5.1 Second locus probed with npi113.
npi202 3L065         Data from Paul Sisco; near vp1.
npi208 4L098     Hin3 21.5, 12 Hin3 15.5, 6.5 0.91 kb fragment from a Pst+H3 digest; there is also a 0.75 kb frag.
npi209A 9L113 Bgl2 5.9 Bgl2 7.5 Eco 5.2 Eco6.0 1.6 kb Hind III insert; 1st locus probed by npi209
npi209B 1S-05 Bgl2 20 Bgl2 13 Eco 8.6 Eco 14.5 2nd band probed by npi209
npi220 8S-48 Bgl2 4.7 ~13 Bgl1 6.9 7.7 <500 bp, HindIII + EcoRI
npi223 6L054 Bgl2 4.2 3.4 Bgl2 4.5 3.5 1.58 kb Hind III insert
npi224A 7S024 EcoRI no allele 20.5 kb     Probable Transposable Element. 10/16/90
npi224B 8L094 EcoRI no allele 15 kb     Probable Transposable Element. 10/16/90
npi224G 1L074     BamHI no allele 20 kb Probable Transposable Element. 10/16/90
npi224I 3S032     BamHI no allele 7.9 kb Probable Transposable Element. 10/16/90
npi225 1L127 Eco 6.4 Eco 11.5, 8.8 Eco 5.6 Eco 7.0 Major band probed with npi98, 1.2 kb H3 insert. 1/14/91
npi235 6S004 Hin3 9.5   Eco 18 24 0.71 kb Pst I insert
npi236 1L084 Eco 17kb Eco 22kb     0.62 kb Pst insert.
npi238 1L147 EcoRI   EcoRV   data from Paul Sisco. 12/14/90
npi239 2S005 Bam 8.2 6.0 Eco 19.5 5.4 B-A 2S confirmed.0.86 kb Pst I insert.
npi242 2L097 BglII 23.1 kb 20.5 kb EcoRI 12.0 kb 14.2 kb 1.15 kb PstI
npi253 9S016 Eco 6.0 19.5 Bam 4.8, 3.8 5.2 0.58 kb Pst I insert.
npi253B 4L097 BamHI   EcoRV   Data from Paul Sisco. 12/14/90
npi253C 5L084 BamHI       Data from Paul Sisco. 12/14/90
npi253D 6L060 BamHI       Data from Paul Sisco. 12/14/90
npi254 2S020 EcoRI   HindIII   Data from Paul Sisco. 12/14/90
npi263 7L068         Data from Paul Sisco. 12/14/90
npi264 10L038 Bam 9.1 16 Bam 8.4, 7.5 5.6 0.77 kb insert.
npi268 8L084 Bam 7.1 5.5 Bam 11 5.6 11 kb Bam is seg`g in COXTx, our version of Tx303 has a 7.2kb band; .71 Kb Pst
npi269A 2S060 Eco 10.6 17.5 Bam 21.5, Hinc2 19 Bam 14, Hinc2 7.0 One of two loci recognized by npi269.PstI 1.0 kb
npi269B 10L049 Eco ~25 3.3     This is a second band recognized by the probe used for NPI269A.PstI 1.0 kb.
npi271 2L094 Hin3 7.8, 4.8 Hin3 7.2, 3.7 Bgl2 10 2.8 B-A not uncovered on 2S.0.62 kb Pst I insert.
npi273 1S-02 BamHI 14.0 kb 13.0 kb BglII 19.5 kb 16.0 kb 1.4 kb EcoRI + HindIII
npi283 7L061 Bgl2 16 9.4 Bgl2 16 13.7 B-A 6L confirmed.0.98 kb Pst I insert.
npi285 10S006 Eco 23 20 Bgl2 2.4 3.3 PstI 1.25 kb
npi288 5L094         Data from Paul Sisco.
npi291 9L115 Bgl2 3.3 Bgl2 3.1 Sst 8.8, 4.9 Sst 8.4, 4.5 1.15 kb PstI insert.
npi297 2L091 EcoRI >23.1 kb,14.8 kb 11.7 kb,7.6 kb     920 bp PstI
npi298 2L147 Bgl2 6.2 14 Bgl2 11.5 6.1 B-A 2L confirmed.0.66 kb Pst I insert.
npi306 10L073 Eco 7.9 9.4 Bgl1 11 Bgl1 24 Near 17.02, 0.84 kb insert cut out with EcoRI+HindIII. First family 16 June '87
npi340A 6S002 EcoRI 15.0 kb 17.5 kb BglII 10.0 kb 6.0 kb Supposed to be 2S (no linkage) & 2 other loci; 1.0 kb PstI; this data near umc85
npi350 10L098         Data from Paul Sisco.
npi361A 2L202 EcoRI no allele 13 kb     Probable Transposable Element. 10/17/90
npi361B 7S020 EcoRI 7.8 kb no allele     Probable Transposable Element. 10/17/90
npi361C 6L038 EcoRI no allele 3.3 kb     Probable Transposable Element. 10/17/90
npi361D 2L169 EcoRI no allele 2.8 kb     Probable Transposable Element. 10/17/90
npi361E 7S025     EcoRI no allele 17.5 kb Probable Transposable Element. 10/17/90
npi361F 8L022     EcoRI 15.6 kb no allele Probable Transposable Element. 10/17/90
npi361I 1L167     EcoRI 3.75 kb no allele Probable Transposable Element. 10/17/90
npi361J 4S016     EcoRI 3.45 kb no allele Probable Transposable Element. 10/17/90
npi361K 2L169     EcoRI 2.85 kb no allele Probable Transposable Element. 10/17/90
npi373 6L018 BamHI       Data from Paul Sisco. 12/14/90
npi400 7S012 Bgl2 13 9.6 Bgl2 9.6 10.5  
npi400B 2L196         Data from Paul Sisco; linked with "pio200075"(now bnl17.14) and pio200581B
npi403 9L112     Eco 7.7 Eco 12.5 0.75 kb PstI insert.
npi404 1S001     Eco 16 Eco 24 2nd locus probed by npi403
npi409 5S-30 Eco 6.7 5.2 Eco 5.75 5.3 0.71 kb Pst I insert.
npi411 1S005 Bam 2.5 7.2 Bam 4.4 7.3 0.98 kb Pst I insert; Veronique estimates 1.2 kb.
npi414 8L097     Bam 12 5.2, 4.4 0.87 kb Pst I insert.
npi421A 2S027 Hin3 6.0 Hin3 7.4 Bgl1 17.5 kb Bgl13.4 kb This is one of two bands recognized by this probe; B-A 2S confirmedPstI 500 bp
npi421B 10L085 Bgl2 ~11 2.2 Bgl11.98 kb 2.7kb Second band probed w/npi421 insert. 2F don't have parents' polymorph.PstI 500 bp
npi425 3L158 Bam 3.7 3.45 Bgl2 5.7 8.6 1.0 kb Pst I insert.
npi427 9L096 Bam 4.8 Bam 4.5 Bam 15, 4.9 Bam 13, 6.6 0.66 kb PstI insert.
npi438 8L108 EcoRI 12.6 kb 9.0 kb EcoRI9.1 kb 16.4 kb 910 bp PstI. 1/18/91
npi443 9L101 Bgl2 4.0, 2.3 Bgl2 3.4, 2.5      
npi445B 10S025 EcoRI 6.5 +/or 5.85 kb 4.0kb BamHI 3.15 kb 2.75 kb 1.02 kb PstI, Supposed to be 5 near centromere.
npi446 3S054 BamHI   HindIII   Data from Paul Sisco. 1/14/91
npi451 4L161 Bgl2 5.6 6.8 Eco >25 19 B-A 4L confirmed.0.62 kb Pst I insert.
npi456 2L111 Bgl2 22.5 10.5 Bgl2 10.5 7.3 B-A 2L confirmed, not 2S.1.31 kb Pst I insert.
npi553 3L066         Data from Paul Sisco.Near 8.35.
npiB1 2S077 BglII 11.5 kb 4.8 kb BglII 7.1 kb 5.3 kb 800 bp EcoRI + HindIII
o2 7S016 Eco 5.9 Eco 6.7 Bgl 7.0 Bgl 8.5 probe is pXho0.9 from R. Schmidt
orp1 4L076         Data from Karen Cone.trpB probe
orp2 10S024         Data from Karen Cone.trpB probe
p1 1S026 - + - + morphological
pcr1 1L103 EcoRI 7.2 kb 6.0 kb     1.32 kb Eco from OR1 cDNA for protochlorophyllide reductase from S. Kay. 12/14/90
pcr2 5S009 EcoRI 4.9 kb 4.2 kb EcoRI 6.8 kb 5.0 kb Probe is EcoRI cut OR1 (1.32 kb) protochlorophyllide reductase cDNA of Steve Kay. 12/14/90
pcr3 2S008 EcoRI >23.1 kb 21.5 kb EcoRI 16.5 kb 23.1 & 17.6 kb 1.32 kb Eco from OR1 cDNA protochlorophyllide reductase of Steve Kay. 12/14/90
pcr4 7S021     EcoRI 14.3 + 13 kb 11 kb Fourth locus probed by protochlorophyllide reductase clone, OR1 from Steve Kay. Dec. 90
pgd1 6L013     2 3.8 isozyme
pgd2 3L071 5 2.8     isozyme
pge2 8L004         Near mdh1; data from Brenda Lowe, transposed Spm.
pge3 7L072         Transposed Spm, data of Brenda Lowe, on top of pio200563
pge14.1 4L112 BamHI 14 kb + 10 kb 11 kb     Data from Brenda Lowe, transposed Spm, probe = 14.1
pge132 1S-24         Data from Brenda Lowe.Kn1 homeodomain homologous sequence. First family only.
pgeA4 8L042         Brenda Lowe: Kn1 homeodomain homologous sequence. Between 2.369 and 12.30.
pgeB5 5S005         Brenda Lowe: Kn1 homeodomain homologous sequence. Vicinity of niu2 and bnl7.56.
pgeC2 1L127         Brenda Lowe: Kn1 homeodomain probed sequence.Same as Kn1?
pgm2 5S000 3 4 3 4 isozyme; supplimentary data from Oliver Nelson.
phi1 1L140 5 4 2 4 isozyme
php1 10S023         Chloroplast phosphoprotein polymorphic in second family.Data of J. Bennett
phy1 1L125 Bgl2 7.7 15 Bgl2 16 7.7 Probe is 1.5 kb Hind3-EcoR fragment from rice phytochrome cDNA pcPhy101, S.A.Kay
phy2 5S-04 Bgl2 4.5 4.9 Bam   same probe as phy1
pio060005 10S024 BglII 12.7 kb 20.5 kb BglII 13 kb 21.8 kb 330 bp PstI
pio060007 6L023     Eco 7.8 or 10 Eco 11.5 350 bp PstI.Tx gave two different kinds of bands - same w/ Bgl2. 28 Nov 90, see pio book 9-5-89
pio100005 9S018 BglII 13.7 kb,5.45 kb 12.5 kb,7.2 kb BglII 22 kb,7.55 kb 14.1 kb,5.8 kb 825 bp PstI
pio100017 5L102 EcoRI 23.1 kb 15.5 kb EcoRI 9.4 kb 18 kb 465 bp PstI
pio100080 3L144 BamHI 16.7 kb,13.3 kb 8.8 kb EcoRI 15 kb 18.5 kb 485 bp PstI
pio200026 3L101 BglII 15 kb 8.4 kb     12/14/90
pio200045 6L022 BglII 13.6 kb 11.2 kb     1.43 kb PstI. 1/18/91
pio200075A 10S-03 BglII 4.2 kb 4.6 kb BglII 4.6 kb 7.4 kb 1.40 kb PstI. 1/18/91
pio200075B 9L060     BglII 2.6 kb 3.0 kb 1.40 kb PstI, Minor band.
pio200523 7L109 BglII 8.3 kb 11.5 kb BglII 9.5 kb 8.6 kb 12/14/90
pio200563 7L072 BamHI 2.22 kb 5.5 kb EcoRI 18.7 kb 13.5 kb 1.15 kb PstI, Dave Grant mapped to 4L terminal.
pio200569A 7L050     BglII 6.1 kb 6.6 kb 1.72 kb PstI, Major band, Supposed to be 7S; weak linkage to 4.24 on 7L.
pio200569B 2L130 BglII 5.25 kb 3.75 kb BglII 5.1 kb 5.65 kb 1.72 kb PstI, Minor band, probed by pio200569.Weak linkage with umc5A on 2L.
pio200581A 7S000 EcoRI 6.3 kb 22.0 kb EcoRI 16.7 kb 9.2 kb 1.40 kb PstI, Lower band
pio200581B 2L194 EcoRI 14.5 kb >23.1 kb EcoRI >23.1 kb 19.3 kb 1.40 kb PstI, Upper band, second band probed by pio200581; linked w/ "pio200075"
pio200599 6L111 BamHI 4.85 kb 4.2 kb EcoRI 23.4 kb 14.5 kb 1.65 kb PstI
pio200626 10S-12 BglII 18.3 kb 8.5 kb EcoRI >23.1 kb 18.7 kb 1.40 kb PstI
pio200690A 7L107 BamHI No band 18 kb BamHI 5.05 kb 7.3 kb 1.43 kb PstI
pio200690B 7S026 BamHI 15 kb No band BamHI No band 15 kb 1.43 kb PstI
pio200713 4S031 BamHI 11.5 kb 4.15 kb BamHI 16.7 kb 10.6kb,4.9 kb 1.47 kb PstI
pio200725 4S030 BamHI 11.5 kb 4.3 kb BamHI 16.5 kb 11 kb,4.9 kb 1.74 PstI
pio200726 3L161 BglII >23.1 kb 20 kb,8 kb BglII 7.5 kb 9.3 kb 1.20 kb PstI
pio200728 7L130 BglII 17.0 kb 13.1 kb EcoRI 6.8 kb 7.2 kb 1.80 kb PstI
pKlamb+ 3L068         Data from Paul Sisco.Combined with abp1 where consistant.
pl1 6L049 Hind III9.7 kb 18.0 kb + - probe is H3-Sal O.6 from K.C. Cone, morphological in 2nd family
r1 10L061 Bgl II 10.3 kb 13.5 kb,8.4 kb     Probe is R5-4 frag.2.
rab17 6L074 EcoRI 11.72 kb 4.95 kb EcoRI 9.1 kb 5.2 kb 400 bp Pst cDNA frag from M. Pages. Recognizes the same frag's as rny1. 10/16/90
rab30 1S-16 BglII 3.35 kb 2.5 kb EcoRI 11.2 kb + 5.8 kb 4.7 kb 400 bp Hind3/EcoRI cDNA frag from M. Pages. Eileen thinks closer to 560 bp. 10/16/90
rny1 6L074 Sst 9.3 Sst 19.5 Bam 8.5 Bam 9.6 Probe is rice rab25 cDNA from J. Mundy.Homologous with rab17 (M. Pages)?
sh1 9S029 Hinc2 ~5 ~ 3 Bgl1 ~14 ~6 probe is Pst38 or Pvu55
sh2 3L149 BamHI 10.5 & 9.5 kb 11.0 & 8.0 kb BamHI 8 & 5.6 kb 10.4 & 4.8 kb 1050 bp Eco. cDNA clone sh2 1050 from Curt Hannah. 1/22/91
stAc 10L030     BglII 5.85 kb 5.55 kb 600 bp H3-Xba fragment flanking Stabilized Ac, Paul Chomet
sus1 9L084     Bam ~11 ~6 Same as Css1, probe is pshD13 from P. Chourey; B-A on 9L
tpi4 3S061 4 N     isozyme
trAc9705 1S-03         Data of Diane Burgess, DNAP.
umc3 3L119 HindIII   HindIII   Data from Paul Sisco. 12/14/90
umc5A 2L122         Data from Paul Sisco.Loosely linked with dia1.
umc5B 7L036         Data from Paul Sisco.
umc15 4L120     BglII 11.25 kb 9.5 kb 750 bp PstI. Tx alleles are 24.5 kb & 6.65 kb in RI Family. No poly in 2F. 1/14/91
umc19 4L137 BglII 11.7 kb 22.5 kb EcoRI >23.1 kb 18.5 kb 670 bp
umc23 1L086         Data from Paul Sisco. 670 bp.
umc31A 4S040         Data from Paul Sisco. 550 bp.; A second locus on 2L.
umc31B 2L157         Data from Paul Sisco, near npi298
umc32A 3S011         data from Paul Sisco
umc32B 8L008         Data from Paul Sisco.
umc33 1L088         Data from Paul Sisco.
umc35 7L137     EcoRI 16.5 kb 13.9 kb 650 bp, PstI
umc37 1L103         Data from Paul Sisco.
umc39 3L121     EcoRV   Data from Paul Sisco. 12/14/90
umc42A 4S068     BamHI 7.0 kb 21.8 kb 640 bp.Data for first family probe a locus on 3L.
umc42B 3S062 BamHI 14.9 kb 10.5 kb     640 bp.Second locus probed with umc42A (4L). Poor data;linked with 6.06 & pdg2
umc43 5S016         Data from Paul Sisco
umc44A 10L055         Data from Paul Sisco.
umc44B 2S041         Data from Paul Sisco
umc47 4S070     BglII 5.9 kb 11.5 kb 740 bp
umc48 8L052         Data from Paul Sisco.
umc49 2L167         Data from Paul Sisco
umc50 3S058         Data from Paul Sisco.
umc51 5L075         Data form Paul Sisco.
umc52 4L113 HindIII       Data from Paul Sisco. 12/14/90
umc53 2S023         data from Paul Sisco
umc54 5L069         Data from Paul Sisco.
umc58 1L068 HindIII   HindIII   Data from Paul Sisco. 12/14/90
umc59 6L017 BamHI 12.8 kb 10.0 kb BamHI 5.7 kb 10.7 kb 930 bp. Tx allele for progeny is 15.7 kb instead of 5.7 kb
umc60 3L073         Data from Pau Sisco.
umc61 2S050 BglII 7.3 kb 8.5 kb BglII 9.4 kb 14 kb Original data from Paul Sisco, near B1. 1.28 kb PstI.
umc64 10L029     BamHI   Data from Paul Sisco. 12/14/90
umc65L 6L042 Bgl2 4.1 Bgl2 7.7     0.65 kb PstI insert
umc66A 5S-13 EcoRI 5.85 kb 10 kb EcoRI 9.0 kb 6.1 kb 1020 bp, Major band, supposed to be on 4S; loose linkage w/ 8.29B on 5S;.5kb2/88. 1F redone 10/16/90
umc66B 1L135 EcoRI 4.85 kb 5.1 kb EcoRI 5.2 kb 5.6 kb 1020 bp, Minor band, probed by umc66.Loose linkage w/ phy1 on 1L in 2nd family. 1F redone 10/16/90
umc72A 5S-02 BamHI       Data from Paul Sisco. 12/14/90
umc72B 1L129 EcoRV       Data from Paul Sisco. 12/14/90
umc84 1L170     HindIII   Data from Paul Sisco. 12/14/90
umc85 6S001         Data from Paul Sisco.
umc95 9L089 BglII 2.9 kb 2.25 kb EcoRI 19.5 kb 8.1 kb 680 bp
umc106 1L126 HindIII   HindIII,EcoRI,Bam   Data from Paul Sisco. 770 bp. 12/14/90
umc119 1L066 HindIII   HindIII   Data from Paul Sisco. 12/14/90
umc122 2L145         Data from Paul Sisco
umc128 1L102         Data from Paul Sisco.
umc130 10S021         Data from Paul Sisco.
umc135 2L108     BamHI   Data from Paul Sisco. 12/14/90
umc147A 5S-10     HindIII   Data from Paul Sisco. 12/14/90
umc147B 1L136     HindIII   Data from Paul Sisco. 12/14/90
umc153 9L072         Data from Paul Sisco. 12/14/90
umc154 3S057 EcoRV       Data from Paul Sisco. 12/14/90
umc155 10S023 EcoRI       Data from Paul Sisco. 12/14/90
umc156 4S071 EcoRI       Data from Paul Sisco. 12/14/90
umc157 1S-15 BamHI   EcoRV   Data from Paul Sisco. 12/14/90
umc158 4L109 EcoRV   EcoRV   Data from Paul Sisco. 12/14/90
umc175 3L066 EcoRV       Data from Paul Sisco. 12/14/90
vp1 3L076 Bgl2 4.3 Bgl2 4.75 Hinc2 4.5 Hinc2 5.1 probe is 0.7 kb Pst of pVPM1B from D. McCarty
wx1 9S056 Bam 5.0, 3.3 4.8 Bam 13, 4.7 4.9. 4.8 probe is pBF225
ynh-me1 3S040         NADP-dependent malic enzyme, chloroplastic leaf form; data of Beverly Rothermel
ynh20 1L120 Hin3 5.9 Hin3 9.4     probe is pBMP-TA69R 1 kb H3-Xho from J. Chen
ynh21 1S027 Bam>25 Bam 15, 6.7 Bam >25 Bam 15, 6.7 probe is pX.5-2 0.4 kb Sst from J. Chen
zp15 6L012 Bam9.4kb 12.5     probe is Eco-H3 insert from pGEMZ14 (Pederson, JBC 261, 6279)
zpB36 7L032 Eco ~12, 8 ~11 Eco ~12 ~16 zein probe
zpL1a 4S029 18 20.5     Zein IEF bands from C. Wilson.
zpL1b 4S031 36 29.5     Zein IEF bands from C Wilson.
zpL1c 4S031 32 31.5     Zein IEF bands from C. Wilson.
zpL1d 4S034 33.5 dark 33.5 light     Zein IEF bands from C. Wilson.
zpL1e 4S030     18, 60 17.5 Zein IEF bands from C. Wilson.; Same as zpL1g.
zpL1f 4S033     32, 36 35 Zein IEF bands from C. Wilson.
zpL2b 7S021     38 21.5, 53 Zein IEF bands from C. Wilson.

3) through 6) the restriction enzyme showing polymorphism and size of the fragment(s) detected segregating with that locus in each of the parental inbreds where known

7) comments on size of the insert, information about the clone and who contributed mapping information

Map positions have negative values where the loci appear to lie distal to 0 on the conventional map.


Please Note: Notes submitted to the Maize Genetics Cooperation Newsletter may be cited only with consent of the authors

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