BROOKINGS, SOUTH DAKOTA

South Dakota State University

Linkage data for opaque endosperm-1 (o1)

--R. H. Whalen

The 1990 working map in MNL 64 indicates that opaque endosperm-1 (o1) lies near gl3 on chromosome 4L, but the order is unknown.

Three point data were obtained from fourteen families of the testcross o1 + gl3 x + Tu +/o1 + gl3, with the following results:
 
Region Genotype No. Totals
0 + Tu + 1799  
  o1 + gl3 1720 3519
1 + + gl3 44  
  o1 Tu + 39 83
2 + Tu gl3 174  
  o1 + + 157 331
1,2 + + + 15  
  o1 Tu gl3 6 21
      3954
o1 - Tu = 2.6±0.3%
Tu - gl3 = 8.9±0.5%

There was no significant heterogeneity among the 14 families for the monohybrid ratios or for linkage. Each of the genes showed good fits to a 1:1 ratio. The data indicate the order to be o1 - Tu - gl3. The coefficient of coincidence was 2.295, due to an excess of the + + + double crossover class. Contamination by foreign pollen is the most likely explanation, since the heterozygote was used as the male parent.


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