UPTON, NEW YORK

Brookhaven National Laboratory

RFLP mapping of mutants

--Eileen C. Matz, Frances A. Burr and Benjamin Burr

We are interested in correlating the RFLP map based on recombinant inbred families T232xCM37 and Tx303xCO159, with the traditional linkage map in regions of the genome where the RFLP map is not marked with common loci. We also have attempted to map two loci that had been mapped only to chromosome arms. In all cases this was done by analyzing the distribution of RFLP alleles in the homozygous mutant class from an F2 population derived from crosses between a full color stock (B, Pl, R-r) and the morphological mutant.
 
Mutant Map position Linked RFLP locus Homozygous recessive class Heterozy-gous class Homozygous dominant class % recomb.
ij2 1L BNL7.25 8/9 1/9 0/9 6
    BNL8.29A 9/9 0/9 0/9 0
    BNL6.32 7/9 2/9 0/9 11
oy1 10S15 PIO200075A 20/31 9/31 2/31 21
    PIO060005 28/31 3/31 0/31 5
py2 1L BNL6.321 4/6 2/6 0/6 17
sr2 10L98 UMC44A 16/18 2/18 0/18 6
    BNL10.13 17/20 2/20 1/20 10
    R1 17/20 3/20 0/20 8
    BNL17.02 17/20 3/20 0/20 8
    GLN1 10/18 7/18 1/18 25
gl15 9L074 BNL5.04 5/5 0/5 0/5 0
    BNL7.13 5/5 0/5 0/5 0

1Most distal probe on 1L; py2 was unlinked with BNL8.29A.


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