--M. G. James, M. J. Scanlon, P. S. Stinard and D. S. Robertson
A large number of defective kernel mutants (deks) have been generated by crosses to Robertson, DS's Mutator for the purpose of transposon tagging. Previously, we reported on the genetic mapping of 47 Mu-induced dek mutants (MNL 65:11) and the determination of allelisms of 17 of these mutants with each other or with previously described kernel mutants (MNL 65:10). In this report, we describe the establishment of 7 additional allelisms (Table 1), provide more detailed and updated mapping information for four previously described mutants (Table 2), and assign 10 new Mu-induced dek mutants to chromosome arm (Table 3). Chromosomal placements were conducted using B-A translocations, the wx-9 series of reciprocal translocations, and allelism tests, and more definitive mapping was achieved with conventional linkage analyses using genetic markers on the appropriate chromosome.
Table 1. Allelic defective kernel mutants.
|Mutant designation||Allelic to:||Chromosome arm||Kernel phenotype||New designation|
|dek*-3252||dek*-2058||4S||crumpled, discolored, lethal||dsc1-3252|
|dek*-5133||sh4||5L||shrunken: collapsed, chalky endosperm||sh4-5133|
|dek*-5132||pro1||8L||crumpled, opaque, lethal||pro1-5132|
|dek*-NS413||dek*-NS326||7L||reduced endosperm, lethal||ren2-413|
|dek*-PI-AEO||dek1||1S||floury endosperm, pigments absent, germless||dek1-PI-AEO|
|Dap2||Dap1||5L||mosaic aleurone color*||Dap1-2|
Table 2. Genetic mapping of Mu-induced defective kernel mutants.
|dek mutant designation||chromosome||linkage testcross||% recombination|
|dek25-2410||4S||Su1 Gl4/Su1 Gl4 x||-su1: 44 +/- 2.39|
|(dek25/su1 gl4)||-gl4: unlinked|
|fl2/fl2 x (fl2/dek25)||-fl2: 25 +/- 1.95a|
|dek7-2608||4S||su1 gl4/su1 gl4 x||-su1: 8.6 +/- 2.28|
|(su1 gl4/dek7)||-gl4: 26.7 +/-3.61|
|fl2/fl2 x (fl2/dek7)||-fl2: 7.1 +/-2.27b|
|dek31-2689||4L||c2/c2 x (c2/dek31)||-c2: 18.6 +/-3.91|
|gl3/gl3 x (gl3/dek31)||-gl3: 19.6 +/- 3.67|
|su1 gl4/su1 gl4 x||-su1: 32 +/- 4.95|
|(su1 gl4/dek31)||-gl4: 22.8 +/- 4.17c|
|emp2||2L||wx/wx x (wx T2-9d/dek*-1047)||-wx T: 21.5 +/- 2.75|
|(dek*-1047)||W3/W3 x (w3/dek*-1047)||-w3: 18.5 +/- 2.43d|
bthe data suggest dek7 - 8.6 - su1 - 18.1 - gl4 and fl2 - 7.1 - dek 7
cthe data suggest su1 - 9 - gl4 - 23 -dek31 - 18.5 - c2
drevised data (from MNL 65:11) suggest emp2 - 18.5 - w3
Table 3. Mu-induced defective kernel mutants placed to chromosome
bmutant was a gift from Nancy Shepherd, DuPont de Nemours, Inc.
We also report that the previous placement by B-A translocation of dek*-326
(renamed ren2, MNL, this issue) to the long arm of chromosome 7
was confirmed through RFLP mapping with MAPMAKER by Lance Veldboom, L and
Mike Lee, M, Iowa State University. dek*-326 was determined to lie
3.2cM distal to BNL14.07 and 4.9cM proximal to BNL8.39.
to the MNL 66 On-Line Index
Return to the Maize Newsletter Index
Return to the Maize Genome Database Page