Callus induction from various genotypes
--Kelly Jo Bates

Independent callus culture lines were generated from over 5000 immature embryos, ranging from 0.8mm to 6.0mm in length. Callus cultures of the following genotypes were established on both MS (Murashige and Skoog) and N6 callus induction media: inbreds A188, A632, B37, B73, CO159, F2, K55, M14, Mo17, N28, Oh43, TANA, Va26, W23, W64a; an Ontario flint; and the hybrid Seneca 60. Embryo age ranged from 9-18 days post pollination; the cultures were maintained at 26 C with a 18h light/6h dark cycle. Callus was characterized according to established (Type I and Type II) criteria (Torné, 1984), or a third class, Type III. Type III consisted of a very distinct sponge-like texture, with an amber to brown appearance.

Table 1. Types of callus observed for the genotypes over a 5 month period of culture.
 
I
II III I & II II&III I&II&III
B37 TANA A632 A188 N28 M14
CO159 Va26 F2 Seneca 60 Mo17
K55 W64A
Oh43 Ontario Flint
W23

Table 2. Preferable media for inducing callus based on responses over a 5 month period of culture.
 
MS N6 BOTH
B73 B37 A188
Mo17 M14 CO159
N28 SENECA 60
W64A TANA
A632 VA26
Ontario Flint F2
K55
Oh43
W23

Regenerated plants from callus over a 5 month period were obtained from: A188, A632, B73, CO159, K55, M14, N28, Oh43, Seneca 60, TANA, W23, W64a; and an Ontario flint. Observations on callus growth are summarized in Tables 1 and 2.

An extensive RFLP analysis is underway on samples of callus and regenerated plants. 


Please Note: Notes submitted to the Maize Genetics Cooperation Newsletter may be cited only with consent of the authors

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