I am using y10 as a cell autonomous marker for mosaic analysis
of lg2, and need to know the distance between y10 and lg2.
Both mutations are recessive, and y10 dies as a white seedling.
Thus, a typical mapping testcross to a double recessive homozygote is not
possible. Robertson, DS mapped y10 approximately 11 map units from
(Robertson, Genetics 46:649-662, 1961). Beckett, JB showed by TB translocations
that y10 is distal to lg2 (J. Hered. 69:27-36, 1978). I obtained
data from selfs of 5 double heterozygotes of lg2 and y10
In order to calculate the recombination frequency, I used two methods, and made the assumption that recombination frequency between these 2 genes is the same in the male and female. Using the product method (Immer, Genetics 15:81-98, 1930), the recombination frequency comes out to 0.134, with a probable error of 1.3%. Using maximum likelihood (Mather, The Measurement of Linkage in Heredity, Wiley and Sons, 2nd ed., 1951), the recombination frequency comes out to 0.135, with a standard error of 1.9%.
Thus y10 is 13.4 map units distal to lg2 on chromosome 3.
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