A new allele, designated sh1-B (shrunken1-Bombay) had been isolated locally which has a somewhat unorthodox breeding behaviour. Upon self-pollination, sh1-B yielded kernels with variable phenotypes. Upon testcrossing to the American tester sh1-A sh1-A all progeny kernels are completely shrunken (Allagikar et al., J. Genet. 70:33, 1991). sh1-A wx/sh1-B Wx heterozygotes were self-pollinated and the progeny kernels were classified for shrunken phenotype (Table 1).
Table 1. Classification of progeny kernels following self-pollination
of plants sh1-A wx/sh1-B Wx.
|Progeny no.||Kernels (shrunken)||Kernels (phenotypically non-shrunk en)||Total||Remarks|
|11:40-1||221||3||224||1 kernel half sectorial for shrunkenness; 1 kernel waxy mutable|
|11:40-2||154||2||156||1 kernel waxy mutable|
It may be inferred: i) some instability prevails at the sh1 locus and ii) two kernels showed typical transposable element-induced instability at the waxy locus.
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