Line 613/2 as a source of a high frequency of spontaneous diploidization
--E. R. Zabirova, O. A. Shatskaya and V. S. Shcherbak
In mass induction of autodiploid lines diploidization of haploid plants becomes one of the problems. Traditionally this is realized by treating the seedlings with 0.12% colchicine solution. In this case a frequency of diploids induced usually ranges from 20 to 30%. Sometimes this value is as high as 50%. However, it is known that the lines passing through a haploid state show a higher percent of diploids. Considering this and the fact that colchicine treatment is a labour consuming process we started searching for a new approach, i.e. a new source of a high frequency of spontaneous diploids. It was found that 33% of the plants originating from 613/2 line in a haploid state became spontaneous diploids (Table 1), in contrast to the lines of the Corn World Collection where no spontaneous diploidization occurred. The selected line was obtained after 4 cycles of selection, i.e. it had passed four times through a haploid state. It seems involvement of the line as a source of a high frequency of diploids into crosses may in part solve the problem of mass induction of haploid lines.
Table 1. Frequency of spontaneous diploids in some corn lines.
|Line||Number of haploids||Number of pollinated ears||Frequency of diploids (%)|
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