Genes encoding methionine-rich proteins: Chromosomal location of a duplicate locus of zps10/(22)
--Todd L. Krone and Ronald L. Phillips

The structural gene for the methionine-rich 10 kDa zein-2, zps10/(22), previously has been mapped to the long arm of chromosome 9. Southern blots of a wide range of maize inbreds, however, indicate that a duplicate locus exists. This highly homologous sequence was cloned by Swarup and Messing (35th Annual Maize Genetics Conference Abstracts, p.12, 1993) and recently designated dzs23. The DNA from 25 F2 individuals from the cross Mo17 x BSSS53 was digested with EcoRI and probed with the clone for zps10/(22) along with a series of probes throughout the maize genome. Segregation analysis indicates dzs23 is located on the long arm of chromosome 6 near umc21, 4 ± 3 cM distal. The dzs23 gene also showed loose linkage with umc65 (24 ± 6 cM). This position is confirmed by Swarup et al. in this issue of the newsletter using a recombinant inbred line population and the dzs23 probe.
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