Chungnam National University
ARS-USDA and University of Missouri

Rindless culm (rlc) gene derived from Dangjin inbred
--Heebong Lee, E.H. Coe, Georgia Davis and Bongho Choe

This study was carried out to identify the location of the gene for rindless culm (rlc), which was derived from Dangjin, a Korean local maize line selfed six generations at the Genetic and Breeding Lab, Coll. of Agri., Chungnam Nat'l. Univ. (Korean J. Breed. 24:42-47, 1992). Materials used were F2s from crosses of waxy translocations with rlc. F2 kernels were separated into waxy and
normal seed, and these were grown in 1994 at Columbia, Missouri. The expression is shown in Figure 1; the plants could be distinguished for rindless culm and normal after tasseling.

Chi-square analysis of the waxy translocation tests indicated that the rindless culm gene is linked to T9-10b (Table 1). All 13 plants from waxy seeds displayed normal culms, while non-waxy seeds showed segregation of normal and rindless culms. This places the rlc gene near T9-10b, which has a breakpoint at 0.4 on the short arm of chromosome 10.

Table 1. Rindless and normal segregations from waxy translocations crossed with Dangjin rindless inbred.
waxy normal
wx T1-9(8389) 6 20 23 34 0.469
wx T4-9b 2 3 2 11 0.242
wx T4-9g 9 23 9 26 0.366x10-8
wx T4-9(5657) 2 17 4 12 0.466
wx T5-9a 9 7 14 13 1.35x10-2
wx T6-9b 5 26 2 35 1.031
wx T7-9(4363) 8 32 11 58 0.076
wx T8-9d 12 22 15 25 2.101x10-3
wx T8-9(6673) 15 17 14 16 0.057
wx T9-10b 0 13 17 17 8.134***
*rlc to normal plant (+) ratio under the assumption of i : j
***significantly deviant at p = 0.001

Figure 1. Rindless culm plant after tasseling. Variable fractions of the culm in the upper few internodes display absence of rind, extending from node to node in sector-like fashion but increasing in degree.

Please Note: Notes submitted to the Maize Genetics Cooperation Newsletter may be cited only with consent of the authors

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