Map locations of Liguleless*-347 and Liguleless*-9167
--Gary J. Muehlbauer, Mike Scanlon and Michael Freeling

Liguleless*-347 (Lg*-347) is a dominant mutation recovered in a Mutator field by Steve Briggs at Pioneer Hi-Bred International Inc. This mutation causes the ligular and basal blade regions near the midrib to be sheath-like, suggesting a transformation of auricle and blade into sheath. Using the waxy reciprocal translocation stocks we mapped Lg*-347 to translocations in the short and long arm of chromosome 3 (Table). DNA gel blot analysis of a segregating Lg*-347 family hybridized to a liguleless3 (lg3) probe (chromosome 3S location; Chi et al., MNL 68:16, 1994) showed no recombinants, indicating that Lg*-347 is located on 3S linked to and possibly allelic to lg3.

Liguleless*-9167 (Lg*-9167) is a spontaneous dominant mutation recovered by Drew Schwartz at the University of Indiana. This mutation also causes the ligular and basal blade regions near the midrib to be sheath-like, suggesting a transformation of auricle and blade into sheath. Using the waxy reciprocal translocation stocks we mapped Lg*-9167 to a translocation in the long arm of chromosome 2 (Table). The map location of this mutation is on the same chromosome arm as Gnarly1 (Gn1; Foster and Hake, MNL 68:2, 1994), indicating that Lg*-9167 may be allelic to Gn1.
 
 
F1 genotype Wx-Lg
(P)
Wx-lg
(R)
wx-Lg
(R)
wx-lg
(P)
Lg*-347
T3-9(8447)wx 
106 7 15 108
Lg*-347 +
T3-9(8562) wx
60 14 3 89
Lg*-9167 +
T2-9d wx
158 55 38 158


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