Cut off tassels from PX-32hh were submerged into 0.1% solution of herbicide basagran (bentason + 2,4DP) for 24h as a pollen test of the herbicide effect. Tassels submerged in tap water were used as control. On the next day five ears from line B-73 were pollinated. Only treated tassels increased the percentage of the obtained haploid seeds by 4-5%. After electrophoretic analysis it was established that these seeds were female and it concerned maternal haploidy.
We conclude that an effect on the high haploid line by 0.1% solution of herbicide basagran can increase the percentage of obtained maternal haploid seeds.
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