A group of multiplasmic lines with Mo17 nucleus, including (Fli) Mo17 (original flint cytoplasm), (su1)Mo17, (sh2)Mo17, (bt1)Mo17, (Pop)Mo17, (wx)Mo17, (Teo)Mo17, (cms-T)Mo17, (cms-S)Mo17, (cms-C)Mo17, and (cms-21A)Mo17 etc., were developed. After crossing, backcrossing was carried out for 24 generations by Mo17 line. The degrees of homo-nucleus of Mo17 had reached 99.99999%. The group of multiplasmic lines could be distinguished by biochemical markers for specific isozymes (or soluble protein, glutaline) bands (or zymograms) (see MNL 71:5-6). The analytic experiments indicated there were almost no differences in morphological characters among these eleven lines, but they were highly different in quantitative characters. Six ear characters in all multiplasmic lines and control (check) (Fli)Mo17 are shown in Table 1. The analysis of variances has shown that the difference in row number per ear and 100grain weight between (su1)Mo17 etc. and control (Fli)Mo17 is not significant, but highly significant in ear length, grain number per row, grain weight per ear and weight per ear. In addition, agronomic characters for F1 (multiplasmic line x Huangzhao si) are shown in Table 2.
F-test demonstrates there are highly significant differences in all experimental characters of all lines, except ear diameter, and highly significant differences also in variety X environment, except ear diameter. This means that the experimental characters are markedly influenced by genetic factors and environment. The ear diameter may be influenced by genetic factors, environment and other factors. See Table 3.
Table 1. Agronomic characters for the multiplasmic line.
Table 2 A & B. Agronomic characters for F1 (multiplasmic line x Huangzhao si).
3. Analysis of variances on agronomic characters for F1 (multiplasmic
line x Huangzhao si).
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