Linkage tests of Non-waxy (Waxy1) reciprocal translocations involving chromosome 9 at the MGCSC
--Jackson, JD, Stinard, P

Approximately 1 acre each year is devoted to the propagation of the large collection of A-A translocation stocks. In this collection is a series of Waxy1-linked translocations that are used for mapping unplaced mutants. Each translocation is maintained in separate M14 and W23 inbred backgrounds which are crossed together to produce vigorous F1's to fill seed requests. Over the years, pedigree and classification problems arose during the propagation of these stocks. We have been able to sort through the problem ones, and we can now supply good sources proven by linkage tests to include the correct translocated chromosomes. Additional translocation stocks will be tested as time allows. Below is a summary of those we have completed testing.

Table 1. Wx1 T3 -9(8562) (3L.65; 9L.22).

A) The M14 sources showed linkage of wx1 with a1.
B) The W23 sources showed linkage of wx1 with a1.

A) 2 point linkage data for a1-Wx1 T3-9(8562)
Testcross: [A1 Wx1 T3-9(8562) x a1 wx1 N] x a1 wx1 N

source:93W-1423-1 ^M14
Region Phenotype No. Totals
0 + Wx 675  
  a wx 656 1331
1 a Wx 174  
  + wx 174 348
% recombination a1-Wx1=2.1±.3

B) 2 point linkage data for a1-Wx1 T3-9(8562)
Testcross: [A1 Wx1 T3-9(8562) x a1 wx1 N] x a1 wx1 N

source:93W-1425-2 ^W23
Region Phenotype No. Totals
0 + Wx 748  
  a wx 607 1355
1 a Wx 193  
  + wx 201 394
% recombination a1-Wx1=2.3±.3

Table 2. Wx1 T4 -9g (4S.27; 9L.27).

A) Some F1's showed linkage of wx1 with su1.
B) Some of the M14 sources showed linkage of wx1 with su1:
C) Some of the W23 sources showed linkage of wx1 with su1:

A) 2 point linkage data for su1-Wx1 T4-9g
Testcross: [Su1 Wx1 T4-9g x su1 wx1 N] x su1 wx1 N

source:87-888 x 990-8 F1 of ^M14 x ^W23
Region Phenotype No. Totals
0 + Wx 1725  
  su wx 1074 2799
1 su Wx 43  
  + wx 92 135
% recombination su1-Wx1=4.6±.3

source:87-889 x 990-8 F1 of ^M14 x ^W23
Region Phenotype No. Totals
0 + Wx 2339  
  su wx 1807 4146
1 su Wx 100  
  + wx 74 174
% recombination su1-Wx1=4.0±.3

B) 2 point linkage data for su1-Wx1 T4-9g
Testcross: [Su1 Wx1 T4-9g x su1 wx1 N] x su1 wx1 N

source:82-215-5^M14
Region Phenotype No. Totals
0 + Wx 1544  
  su wx 1212 2756
1 su Wx 30  
  + wx 48 78
% recombination su1-Wx1=2.8±.3

source:84-15-2^M14
Region Phenotype No. Totals
0 + Wx 290  
  su wx 234 524
1 su Wx 6  
  + wx 5 11
% recombination su1-Wx1=2.1±.6

source:84-15-4^M14
Region Phenotype No. Totals
0 + Wx 1123  
  su wx 896 2019
1 su Wx 35  
  + wx 56 91
% recombination su1-Wx1=4.3±.4

source:79-105-5^M14
Region Phenotype No. Totals
0 + Wx 870  
  su wx 718 1588
1 su Wx 28  
  + wx 28 56
% recombination su1-Wx1=3.4±.4

C) 2 point linkage data for su1-Wx1 T4-9g
Testcross: [Su1 Wx1 T4-9g x su1 wx1 N] x su1 wx1 N

source:93W-1443-3^W23
Region Phenotype No. Totals
0 + Wx 275  
  su wx 217 492
1 su Wx 10  
  + wx 15 25
% recombination su1-Wx1=4.8±.9

Table 3. Wx1 T5-9c (5S.07; 9L.10).

A) The M14 source showed linkage of wx1 with a2.
B) The W23 source showed no linkage of wx1 with a2.

A) 2 point linkage data for a2-Wx1 T5-9c
Testcross: [A2 Wx1 T5-9c x a2 wx1 N] x a2 wx1 N

source:82-142-3 ^M14
Region Phenotype No. Totals
0 + Wx 1545  
  a wx 1359 2904
1 a Wx 65  
  + wx 66 131
% recombination a2-Wx1=4.3±.4

Table 4. Wx1 T5-9(4817) (5L.06; 9S.07).

A) The M14 source showed linkage of wx1 with a2:
B) Some W23 sources showed linkage of wx1 with a2.

A) 2 point linkage data for a2-Wx1 T5-9(4817)
Testcross: [A2 Wx1 T5-9(4817) x a2 wx1 N] x a2 wx1 N

source:93W-1445-3 ^M14
Region Phenotype No. Totals
0 + Wx 343  
  a wx 406 749
1 a Wx 15  
  + wx 19 34
% recombination a2-Wx1=4.3±.7

source:93W-1445-3 ^M14
Region Phenotype No. Totals
0 + Wx 912  
  a wx 886 1798
1 a Wx 65  
  + wx 47 112
% recombination a2-Wx1=5.9±.5

B) 2 point linkage data for a2-Wx1 T5-9(4817)
Testcross: [A2 Wx1 T5-9(4817) x a2 wx1 N] x a2 wx1 N

source:84-07-8 ^W23
Region Phenotype No. Totals
0 + Wx 1796  
  a wx 1696 3492
1 a Wx 96  
  + wx 97 193
% recombination a2-Wx1=5.2±.4

source:85-019-1 ^W23
Region Phenotype No. Totals
0 + Wx 487  
  a wx 489 976
1 a Wx 11  
  + wx 16 27
% recombination a2-Wx1=2.7±.5

Table 5. Wx1 T9-10(8630) (9S.28; 10L.37).

A) All of the M14 sources showed no linkage of wx1 with either r1 or g1.
B) Some of the W23 sources showed linkage of wx1 with r1 & g1:

B) 2 point linkage data for r1-Wx1 T9-10(8630)
Testcross: [r1 Wx1 T9-10(8630) x R1 wx1 N] x r1 wx1 N

source:93W-1459-5^W23
Region Phenotype No. Totals
0 r Wx 1187  
  + wx 992 2179
1 r wx 157  
  + Wx 377 534
% recombination r1-Wx1=19.7±.7

B) 2 point linkage data for g1-Wx1 T9-10(8630)
Testcross: [G1 Wx1 T9-10(8630) x g1 wx1 N ] x g1 wx1 N

source:93W-1459-5^W23
Region Phenotype No. Totals
0 + Wx 761  
  g wx 755 1516
1 g Wx 47  
  + wx 25 72
% recombination g1-Wx1=4.5±.5


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