Linkage tests of waxy1 marked reciprocal translocations at the MGCSC
--Jackson, JD, Stinard, P

In the collection of A-A translocation stocks maintained at MGCSC is a series of waxy1-linked translocations that are used for mapping unplaced mutants. Also new wx1-linked translocations are being introduced into this series and are in a conversion program to convert each translocation to the inbred backgrounds M14 and W23. These inbreds are then crossed together to produce vigorous F1's to fill seed requests. Over the years, pedigree and classification problems arose during the propagation of these stocks. We have been able to sort through the problem ones, and we can now supply good sources proven by linkage tests to include the correct translocated chromosomes. Additional translocation stocks will be tested as time allows. Below is a summary of those we have completed testing. Additional pedigree information on bad sources is available should anyone want to check on sources supplied to them by the Stock Center.

Table 1. wx1 T3 -9(8562) (3L.65; 9L.22) (COOP source).

A) The M14 source showed no linkage of wx1 with a1.
B) The W23 source showed linkage of wx1 with a1.

B) 2 point linkage data for a1-wx1 T3-9(8562)
Testcross: [A1 wx1 T3-9(8562) x a1 Wx1 N] x a1 wx1 N

source:82-087-3 ^W23
Region Phenotype No. Totals
0 a Wx 2292  
  + wx 2019 4311
1 + Wx 816  
  a wx 857 1673
% recombination a1-Wx1=2.8±.2

Table 2. wx1 T3 -9(8562) (new source from D. Robertson).

2 point linkage data for a1-Wx1 T3-9(8562)
Testcross: [A1 wx1 T3-9(8562) x a1 Wx1 N] x a1 wx1 N

source:93W-1477-6
Region Phenotype No. Totals
0 a Wx 1328  
  + wx 906 2234
1 + Wx 458  
  a wx 397 855
% recombination a1-Wx1=2.8±.3

Table 3. wx1 T5-9c (5S.07; 9L.10).

A) The M14 source showed no linkage of wx1 with a2.
B) The W23 source showed linkage of wx1 with a2.

B) 2 point linkage data for a2-wx1 T5-9c
Testcross: [A2 wx1 T5-9c xa2 Wx1 N ] x a2 wx1 N

source:92-411-1 ^W23
Region Phenotype No. Totals
0 a Wx 1270  
  + wx 1101 2371
1 + Wx 142  
  a wx 74 216
% recombination a2-Wx1=8.3±.5

Table 4. wx1 T5-9(8854) (55.33; 9S.36).

This source showed no linkage of wx1 with a2:

Table 5. wx1 T5-9(022-11) (55.30; 9L.27).

2 point linkage data for a2-wx1 T5-9(022-11)
Testcross: [A2 wx1 T5-9(022-11) x a2 Wx1 N] x a2 wx1 N

source:93-2128-1-
Region Phenotype No. Totals
0 a Wx 1978  
  + wx 1686 3664
1 + Wx 91  
  a wx 53 144
% recombination a2-Wx1=3.8+.3

Table 6. wx1 T9-10b (9S.13; 10S.40).

A) The M14 sources showed linkage of wx1 with bf2.
B) The W23 sources showed linkage of wx1 with bf2.

A) 2 point linkage data for bf2-wx1 T9-10b
Testcross: [Bf2 wx1 T9-10b x bf2 Wx1 N] x bf2 wx1 N

source:82-116-1^M14
Region Phenotype No. Totals
0 bf Wx 334  
  + wx 312 646
1 + Wx 46  
  bf wx 19 65
% recombination r1-Wx1=9.1+1.1

B)2 point linkage data for bf2-wx1 T9-10b
Testcross: [Bf2 wx1 T9-10b x bf2 Wx1 N] x bf2 wx1 N

source:82-117-1^W23
Region Phenotype No. Totals
0 bf Wx 812  
  + wx 892 1704
1 + Wx 40  
  bf wx 39 79
% recombination r1-Wx1=4.4+.5

Table 7. wx1 T1 9-10(059-10 (9S.31; 10L.53).

2 point linkage data for r1-wx1 T9-10(059-10)
Testcross: [r1 wx1 T9-10(059-10) x R1 Wx1 N] x r1 wx1 N

source:93-2069-1
Region Phenotype No. Totals
0 + Wx 165  
  r wx 362 527
1 r Wx 6  
  + wx 37 43
% recombination r1-Wx1=7.5+1.1

Table 8. wx1 T1 9-10(8630) (9S.28; 10L.37).

All of the wx1 T9-10(8630) sources showed no linkage of wx1 with either r1 or g1.

A new potential crossover has been identified.


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