VI. MAIZE PROBE BANK
DNA clones in the public bank and distribution center at the UMC Maize RFLP Lab now total ~ 6700, of which we distribute more than 4900 (Table 1). This includes the large sets of sequenced cDNAs generated by Chris Baysdorfer, California State University - Hayward (designated csu), and by Tim Helentjaris (designated uaz or 5C, 6C, 7C), which represent the largest collection of candidate genes publicly available for maize. Over the last five years the UMC Maize Probe Bank has filled more than 820 requests for more than 24,000 clones to 31 countries, including 87 sets of the UMC Core Marker probes, which have become the standard landmark probe set for mapping genes, phenotypes and QTLs in maize.
The activities of the RFLP Lab and the Probe Bank are meaningful as they contribute to the research needs of the community. The easiest avenue to request clones is through the "PROBE Requests" form in MaizeDB, http://www.agron.missouri.edu, or by email to firstname.lastname@example.org.
To contribute clones for distribution or to be mapped onto the UMC Maize Genetic Map contact Theresa Musket at the email listed above and she will inform you of the information and materials needed.
These activities are supported by USDA-ARS CRIS projects "Genetic Mechanisms and Molecular Genetic Resources for Corn" and "Maize Genome Database"; and by FAO/IAEA Coordinated Research Program, "Molecular Markers for Maize, Rye and Rice".
Table 1. Clones maintained by UMC RFLP Lab and Probe Bank.
|Clone Set||Abbreviation||Type of Clone||Total Number in Set||Distribution by UMC|
|Brookhaven National Laboratories||bnl||maize genomic||109||Yes|
|Brookhaven National Laboratories||bnl||maize cDNA||12||Yes|
|California State University-Hayward||csu||maize cDNA||1197||Yes|
|Contributed Clones||mostly maize||295||90 Yes, 205 No|
|Cornell University||bcd, cdo, rg, rz||barley, oat, rice||151||No|
|Iowa State University||isu||maize cDNA||136||Yes|
|Mycogen Plant Sciences||agr||maize cDNA||413||Yes|
|Pioneer Hi-Bred International||php||maize genomic||161||Yes|
|Pioneer Hi-Bred International||npi||maize both||236||Yes|
|Rice Genome Research Program||rgp||rice||371||No|
|Texas A&M University||txs||sorghum genomic||145||No|
|University of Arizona||uaz 5C, 6C, 7C||maize cDNA||1920||Yes|
|University of Hohenheim||Ch6S||maize genomic||339||No|
|University of Minnesota||umn||oat||29||No|
|University of Missouri||umc||maize both||249||237 Yes, 12 No|
|University of Missouri-Rice||pOs||rice genomic||18||Yes|
|University of Missouri-Rye||ScG||rye genomic||171||Yes|
|University of Missouri-Tripsacum||tda||tripsacum genomic||476||20 Yes, 456 No|
|Total Number of Clones:||6694|
|Total Distributable Clones:||4906|
Theresa Musket, Mary Polacco, Mike McMullen, and Ed Coe
TWO BULLETINS ABOUT THE DOG
Following note was received from a Fish person:
the dog's name was found on a
fishing trip, underwent
horizontal transfer, can
be found hob-knobbing
at a new location.
(the reward is a hank of clothesline, toward future fishing for larger games and toward keeping the dog running).
Received documentation from Day that calls for reopening play; investigations are underway, dog's name is subject to re-assay.
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