Additional linkage tests of non-waxy (Waxy1) reciprocal translocations involving chromosome 9 at the MGCSC
--Jackson, JD, Stinard, P

Approximately 1 acre each year is devoted to the propagation of the large collection of A-A translocation stocks. In this collection is a series of Waxy1-linked translocations that are used for mapping unplaced mutants. Each translocation is maintained in separate M14 and W23 inbred backgrounds which are crossed together to produce vigorous F1's to fill seed requests. Over the years, pedigree and classification problems arose during the propagation of these stocks. We have been able to sort through the problem ones, and we can now supply good sources proven by linkage tests to include the correct translocated chromosomes. Below is a summary of additional translocation stocks we have completed testing.

Table 1. Wx1 T2-9b F1 (2S.18; 9L.22)

A) The F1 source showed linkage of wx1 with v4:

2 point linkage data for v4-Wx1 T2-9b
Testcross: [V4 Wx1 T2-9b x v4 wx1 N] x v4 wx1 N

source:87-966 x 964 F1 of ^M14 x ^W23
Region Phenotype No. Totals
0 + Wx 1629  
  v wx 1342 2971
1 v Wx 205  
  + wx 175 380
% recombination v4-Wx1= 11.3+.5

------------------------------------------------------------

Table 2. Wx1 T5-9d (5L.14; 9L.10)

A) The M14 sources showed linkage of wx1 with gl8:

2 point linkage data for gl8-Wx1 T5-9d
Testcross: [Gl8 Wx1 T5-9d x gl8 wx1 N] x gl8 wx1 N

source:93W-1449 ^M14
Region Phenotype No. Totals
0 + Wx 58  
  gl wx 42 100
1 gl Wx 15  
  + wx 24 39
% recombination gl8-Wx1= 28.1+3.8

source:94-1867-1 ^M14
Region Phenotype No. Totals
0 + Wx 497  
  gl wx 492 989
1 gl Wx 129  
  + wx 131 260
% recombination gl8-Wx1= 20.8+1.1

source:94-1869-1 ^M14
Region Phenotype No. Totals
0 + Wx 1332  
  gl wx 1282 2614
1 gl Wx 331  
  + wx 272 603
% recombination gl8-Wx1= 18.7+.7

B) The W23 sources showed no linkage of wx1 with gl8.

------------------------------------------------------------

Table 3. Wx1 T7-9(4363) (7ctr.; 9ctr.)

A) Some M14 sources showed linkage of wx1 with gl1:

2 point linkage data for gl1-Wx1 T7-9(4363)
Testcross: [gl1 Wx1 T7-9(4363) x Gl1 wx1 N] x gl1 wx1 N

source:94-1874 ^M14
Region Phenotype No. Totals
0 gl Wx 98  
  + wx 101 199
1 + Wx 13  
  gl wx 8 21
% recombination gl1-Wx1=9.5+2.0

source:95-889-2 ^M14
Region Phenotype No. Totals
0 gl Wx 931  
  + wx 942 1873
1 + Wx 49  
  gl wx 35 84
% recombination gl1-Wx1=4.3+.5

source:76-2062-1 ^M14
Region Phenotype No. Totals
0 gl Wx 428  
  + wx 434 862
1 + Wx 36  
  gl wx 12 48
% recombination gl1-Wx1=5.3+.7

B) Some W23 sources showed linkage of wx1 with gl1:

2 point linkage data for gl1-Wx1 T7-9(4363)
Testcross: [gl1 Wx1 T7-9(4363) x Gl1 wx1 N] x gl1 wx1 N

source:87-1029 ^W23
Region Phenotype No. Totals
0 gl Wx 663  
  + wx 731 1394
1 + Wx 19  
  gl wx 12 31
% recombination gl1-Wx1=2.2+.4

source:94-1876 ^W23
Region Phenotype No. Totals
0 gl Wx 612  
  + wx 622 1234
1 + Wx 38  
  gl wx 18 56
% recombination gl1-Wx1=4.3+.6

------------------------------------------------------------

Table 4. Wx1 T9-10(8630) (9S.28; 10L.37)

A) Unconverted source showed linkage of wx1 with r1:

2 point linkage data for r1-Wx1 T9-10(8630)
Testcross: [r1 Wx1 T9-10(8630) x R1 wx1 N] x r1 wx1 N

source:72-3342-2
Region Phenotype No. Totals
0 r Wx 935  
  + wx 850 1785
1 r wx 120  
  + Wx 174 294
% recombination r1-Wx1=14.1+.8

B) Additional W23 sources showed linkage of wx1 with r1:

2 point linkage data for r1-Wx1 T9-10(8630)
Testcross: [r1 Wx1 T9-10(8630) x R1 wx1 N] x r1 wx1 N

source:87-1077 ^W23
Region Phenotype No. Totals
0 r Wx 1445  
  + wx 1085 2530
1 r wx 172  
  + Wx 250 422
% recombination r1-Wx1=14.3+.6

source:93W-1459-5 ^W23
Region Phenotype No. Totals
0 r Wx 1052  
  + wx 736 1788
1 r wx 117  
  + Wx 192 309
% recombination r1-Wx1=14.7+.8

source:93W-1459-6 ^W23
Region Phenotype No. Totals
0 r Wx 1213  
  + wx 910 2123
1 r wx 136  
  + Wx 248 384
% recombination r1-Wx1=15.3+.7
 


Please Note: Notes submitted to the Maize Genetics Cooperation Newsletter may be cited only with consent of the authors.

Return to the MNL 73 On-Line Index
Return to the Maize Newsletter Index
Return to the Maize Genome Database Page