Additional linkage tests of waxy1 marked reciprocal translocations at the MGCSC
--Jackson, JD, Stinard, P

In the collection of A-A translocation stocks maintained at MGCSC is a series of waxy1-linked translocations that are used for mapping unplaced mutants. Also new wx1-linked translocations are being introduced into this series and are in a conversion program to convert each translocation to the inbred backgrounds M14 and W23. These inbreds are then crossed together to produce vigorous F1's to fill seed requests. Over the years, pedigree and classification problems arose during the propagation of these stocks. We have been able to sort through the problem ones, and we can now supply good sources proven by linkage tests to include the correct translocated chromosomes. Additional translocation stocks will be tested as time allows. Below is a summary of additional translocation stocks we have completed testing. Additional pedigree information on bad sources is available should anyone want to check on sources supplied to them previously by the Stock Center.

Table 1. wx1 T2-9b (2S.18; 9L.22)

A) The M14 source showed no linkage of wx1 with v4.

B) The W23 source showed linkage of wx1 with v4:

2 point linkage data for v4-wx1 T2-9b
Testcross: [V4 wx1 T2-9b x v4 Wx1 N] x v4 wx1 N

source:82-082-2 ^W23
Region Phenotype No. Totals
0 v Wx 1087  
  + wx 994 2081
1 + Wx 225  
  v wx 141 366
% recombination v4-Wx1=15.0+.7

------------------------------------------------------------

Table 2. wx1 T5-9a (5L.69; 9S.17)

A) The M14 source showed linkage of wx1 with v2:

2 point linkage data for v2-wx1 T5-9a
Testcross: [V2 wx1 T5-9a x v2 Wx1 N] x v2 wx1 N

source:92H-475-2 ^M14
Region Phenotype No. Totals
0 v Wx 501  
  + wx 571 1072
1 + Wx 57  
  v wx 48 105
% recombination v2-Wx1=8.9+.8

B) The W23 source showed linkage of wx1 with v2:

2 point linkage data for v2-wx1 T5-9a
Testcross: [V2 wx1 T5-9a x v2 Wx1 N] x v2 wx1 N

source:92H-474-2 ^W23
Region Phenotype No. Totals
0 v Wx 578  
  + wx 677 1255
1 + Wx 57  
  v wx 45 102
% recombination v2-Wx1=7.5+.7

------------------------------------------------------------

Table 3. wx1 T6-9b (6L.10; 9S.37)

A) The M14 sources showed linkage of wx1 with y1:

2 point linkage data for y1-wx1 T6-9b
Testcross: [Y1 wx1 T6-9b x y1 Wx1 N] x y1 wx1 N

source:88-976 x 977 ^M14
Region Phenotype No. Totals
0 y Wx 2160  
  + wx 1219 3379
1 + Wx 77  
  y wx 43 120
% recombination y1-Wx1=3.4+.3

2 point linkage data for y1-wx1 T6-9b
Testcross: [y1 wx1 T6-9b x Y1 Wx1 N] x y1 wx1 N

source:87-1711-3 ^M14
Region Phenotype No. Totals
0 + Wx 1755  
  y wx 1181 2936
1 y Wx 25  
  + wx 19 44
% recombination y1-Wx1=1.5+.2

B) The W23 sources showed linkage of wx1 with y1:

2 point linkage data for y1-wx1 T6-9b
Testcross: [y1 wx1 T6-9b x Y1 Wx1 N] x y1 wx1 N

source: 94-1907-1^W23
Region Phenotype No. Totals
0 + Wx 1403  
  y wx 1093 2496
1 y Wx 29  
  + wx 29 58
% recombination y1-Wx1=2.3+.3

source:97-1275-1;-2;-4 ^W23
Region Phenotype No. Totals
0 + Wx 993  
  y wx 843 1836
1 y Wx 42  
  + wx 50 92
% recombination y1-Wx1=4.8+.5

source:88-972-974 Bulk 1 ^W23
Region Phenotype No. Totals
0 + Wx 1400  
  y wx 881 2281
1 y Wx 36  
  + wx 44 80
% recombination y1-Wx1=3.4+.4

------------------------------------------------------------

Table 4. wx1 T7-9(4363) (7ctr.; 9ctr.)

A) The M14 sources showed no linkage of wx1 with gl1.

B) The W23 sources showed linkage of wx1 with gl1:

2 point linkage data for gl1-wx1 T7-9(4363)
Testcross: [gl1 wx1 T7-9(4363) x Gl1 Wx1 N] x gl1 wx1 N

source:88-980 x 979 ^W23
Region Phenotype No. Totals
0 + Wx 1737  
  gl wx 1780 3517
1 gl Wx 150  
  + wx 155 305
% recombination gl1-Wx1=8.0+.4

source:93W-1416 ^W23
Region Phenotype No. Totals
0 + Wx 450  
  gl wx 442 892
1 gl Wx 28  
  + wx 29 57
% recombination gl1-Wx1=6.0+.8

------------------------------------------------------------

Table 5. wx1 T7-9a (7L.63.; 9S.07)

A) The M14 source showed linkage of wx1 with gl1:

2 point linkage data for gl1-wx1 T7-9a
Testcross: [gl1 wx1 T7-9a x Gl1 Wx1 N] x gl1 wx1 N

source:92-429-2 ^M14
Region Phenotype No. Totals
0 gl Wx 832  
  + wx 768 1600
1 + Wx 127  
  gl wx 135 262
% recombination gl1-Wx1=14.1+.8

B) The W23 source showed linkage of wx1 with gl1:

2 point linkage data for gl1-wx1 T7-9a
Testcross: [gl1 wx1 T7-9a x Gl1 Wx1 N] x gl1 wx1 N

source:92H-482-1 ^W23
Region Phenotype No. Totals
0 gl Wx 1120  
  + wx 1188 2308
1 + Wx 245  
  gl wx 203 448
% recombination gl1-Wx1=16.3+.7
 


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