Institute of Genetics, Bulgarian Academy of Sciences
Table 1. Regeneration frequencies of
A619 x M320 or M320 x A619 hybrids.
|Genotype||Number of embryos with morphogenesis||Number of regenerated plants|
|A619 x M320
M320 x A619
The results differed in dependence on the line used as a female parent. It is obvious that maternal genotype A619 had a valuable effect on the frequency of plants produced in vitro.
We speculate that a factor determining these results may be the importance of combination between nucleus and cytoplasm. It is true for the case where A619 is the female partner, which is not the situation with A619 being the male partner, where there is only a nucleus.
The viability of regenerated plants was not high. During development part of the plants did not elongate and their appearance was like that established by Pilu; et al. (MNL 73:69). Future experiments will elucidate differentiation in other traits of hybrids obtained in a "classical crosses" way.
This work was supported by grant B-602
from the National Fund of Scientific Investigations of the Bulgarian Ministry
of Education and Science.
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