Institute of Genetics, Bulgarian Academy of Sciences

Studying the possibilities for transfer of regeneration capability in vitro via classical crosses --Nedev, T, Kruleva, M, Krapchev, B We have previously reported (MNL 72:76) about good regeneration capability of in vitro culture of inbred maize line A619. The protocol of embryo isolation, media used, and the in vitro tissue culture procedures were published there too. The aim of the present work was to verify the possibility of transferring the trait "good capability" from line A619 to hybrid combinations A619 x M320 or M320 x A619 (A619 as a female and male partner). The results obtained for the two combinations are presented in Table 1.

Table 1. Regeneration frequencies of A619 x M320 or M320 x A619 hybrids.
Genotype Number of embryos with morphogenesis Number of regenerated plants
A619 x M320

M320 x A619





The results differed in dependence on the line used as a female parent. It is obvious that maternal genotype A619 had a valuable effect on the frequency of plants produced in vitro.

We speculate that a factor determining these results may be the importance of combination between nucleus and cytoplasm. It is true for the case where A619 is the female partner, which is not the situation with A619 being the male partner, where there is only a nucleus.

The viability of regenerated plants was not high. During development part of the plants did not elongate and their appearance was like that established by Pilu; et al. (MNL 73:69). Future experiments will elucidate differentiation in other traits of hybrids obtained in a "classical crosses" way.

This work was supported by grant B-602 from the National Fund of Scientific Investigations of the Bulgarian Ministry of Education and Science.

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