CHESTNUT HILL, MASSACHUSETTS
Continued studies on mutable inbred
derived from anther culture
--Ting, YC, Tran, L
In the last summer continued studies on
the mutable inbred derived from anther culture of maize KH-13 were carried
out. This inbred was a descendant from a single self-fertilized plant which
was dark green in plant color and normal in fertility for both male and
female flowers. A total of 120 kernels was sown in the field and from them
102 plants grew into adult stage. However, it was observed that when the
plants were about two-months old, approximately 10 percent, nine plants,
appeared slow in growth and short in height. About two weeks later, these
short plants became dwarf yellow-green. Apparently their leaf chlorophylls
were deficient. All except one, of these plants had barren stalk and sterile
male inflorescence. The exceptional plant developed a small ear and a limited
amount of pollen. Upon self-pollination, seven defective kernels were obtained.
A test on their viability will be made in the next season. As was stated
above, nine of 102 plants were phenotypically dwarf yellow-green. According
to the Mendelian segregation principle, this was unexpected. It does not
fit the expected segregation ratio of either monohybrids or dihybrids.
Hence, a hypothesis was once again proposed that the unconventional segregation
ratio was evidence of the presence of an Ac element in the parental
plant. This element was activated through anther culture per se.
Please Note: Notes submitted to the Maize Genetics Cooperation Newsletter may be cited only with consent of the authors.
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