Urbana, Illinois

Maize Genetics Cooperation Stock Center

Additional linkage tests of non-waxy (Waxy1) reciprocal translocations involving chromosome 9 at the MGCSC --Jackson, JD, Stinard, P, Zimmerman, S Approximately 1 acre each year is devoted to the propagation of the large collection of A-A translocation stocks. In this collection is a series of Waxy1-linked translocations that are used for mapping unplaced mutants. Each translocation is maintained in separate M14 and W23 inbred backgrounds which are crossed together to produce vigorous F1's to fill seed requests. Over the years, pedigree and classification problems arose during the propagation of these stocks. We have been able to sort through the problem ones, and can now supply good sources proven by linkage tests to include the correct translocated chromosomes.

Previously we reported the linkage results for some of these stocks (MNL72:79-81; MNL73:86-88). Below is a summary of additional translocation stocks we have completed testing.

Table 1. Wx1 T1-9(4995) (1L.19; 9S.20)

A) The M14 source showed linkage of wx1 with bz2.

2 point linkage data for bz2-Wx1 T1-9(4995)

Testcross: [Bz2 Wx1 1-9(4995) x bz2 wx1 N] x bz2 wx1 N

source:94-1863-2^M14
 
Region Phenotype No. Totals
0 + Wx 581  
  bz wx 537 1118
1 bz Wx 138  
  + wx 176 314

% recombination bz2-Wx1= 21.9+1.1

B) The W23 source showed linkage of wx1 with f1 & bz2.

1) 2 point linkage data for bz2-Wx1 T1-9(4995)

Testcross: [Bz2 Wx1 1-9(4995) x bz2 wx1 N] x bz2 wx1 N

source:94-1865-3^W23
 
Region Phenotype No. Totals
0 + Wx 485  
  bz wx 425 910
1 bz Wx 148  
  + wx 115 263

% recombination bz2-Wx1= 22.4+1.2

2) 2 point linkage data for f1-Wx1 T1-9(4995)

Testcross: [F1 Wx1 1-9(4995) x f1 wx1 N] x f1 wx1 N

source:94-1865-3^W23
 
Region Phenotype No. Totals
0 + Wx 1919  
  f wx 1726 3645
1 f Wx 327  
  + wx 251 578

% recombination f1-Wx1= 13.7+0.5

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Table 2. Wx1 T4-9e (4S.53; 9L.26)

A) The M14 source showed linkage of wx1 with su1:

2 point linkage data for su1-Wx1 T4-9e

Testcross: [Su1 Wx1 T4-9e x su1 wx1 N] x su1 wx1 N

source:87-987 / 986 Bulk ^M14
 
Region Phenotype No. Totals
0 + Wx 788  
  su wx 684 1472
1 su Wx 81  
  + wx 22 103

% recombination su1-Wx1= 6.5+0.6

B) The W23 source showed linkage of wx1 with su1.

2 point linkage data for su1-Wx1 T4-9e

Testcross: [Su1 Wx1 T4-9e x su1 wx1 N] x su1 wx1 N

source:87-985 x sib ^W23
 
Region Phenotype No. Totals
0 + Wx 840  
  su wx 782 1622
1 su Wx 194  
  + wx 34 228

% recombination su1-Wx1= 12.3+0.8

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Table 3. Wx1 T9-10b (9S.13; 10S.40)

A) The F1 source showed linkage of wx1 with bf2:

2 point linkage data for bf2-Wx1 T9-10b

Testcross: bf2 wx1 N x [Bf2 Wx1 T9-10b x bf2 wx1 N]

source:87-1050 / 1049 bulk (1)
 
Region Phenotype No. Totals
0 + Wx 729  
  bf wx 610 1339
1 bf Wx 39  
  + wx 41 80

% recombination bf2-Wx1=5.6+0.6
 
 


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