Additional linkage tests of waxy1 marked reciprocal translocations at the MGCSC --Jackson, JD, Stinard, P, Zimmerman, S In the collection of A-A translocation stocks maintained at MGCSC is a series of waxy1-linked translocations that are used for mapping unplaced mutants. Also new wx1-linked translocations are being introduced into this series and are in a conversion program to convert each translocation to the inbred backgrounds M14 and W23. These inbreds are then crossed together to produce vigorous F1's to fill seed requests. Over the years, pedigree and classification problems arose during the propagation of these stocks. We have been able to sort through the problem ones, and can now supply good sources proven by linkage tests to include the correct translocated chromosomes. Additional pedigree information on bad sources is available should anyone want to check on sources supplied to them previously by the Stock Center.

Previously we reported the linkage results for some of these stocks (MNL72:81-82; MNL73:88-89). Below is a summary of additional translocation stocks we have completed testing. Additional translocation stocks will be tested as time allows.

Table 1. wx1 T1-9c (1S.48; 9L.22)

A) The W23 source showed linkage of wx1 with P1-ww:

2 point linkage data for P1-ww-wx1 T1-9c

Testcross: [P1-wr Wx1 N x P1-ww wx1 T1-9c] x P1-ww wx1 N wx1

source:94-1891-1^W23
 
Region Phenotype No. Totals
0 P1-wr Wx 62  
  P1-ww wx 72 134
1 P1-ww Wx 10  
  P1-wr wx 3 13

% recombination P1-ww-Wx1=8.8+2.3

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Table 2. wx1 T1-9(4995) (1L.19; 9S.20) COOP source

A) The new M14 c/o sources showed linkage of wx1 with bz2 & f1.

1) 2 point linkage data for bz2-wx1 T1-9(4995)

Testcross: [Bz2 wx1 1-9(4995) x bz2 wx1 N] x bz2 wx1 N

source:94-1845-1c/o^M14
 
Region Phenotype No. Totals
0 bz Wx 595  
  + wx 693 1288
1 + Wx 222  
  bz wx 191 413

% recombination bz2-Wx1= 24.3+1.0

Testcross: bz2 wx1 N x [bz2 wx1 N x Bz2 wx1 1-9(4995)]

source:94-1845-2c/o^M14
 
Region Phenotype No. Totals
0 bz Wx 71  
  + wx 82 153
1 + Wx 19  
  bz wx 37 56

% recombination bz2-Wx1= 26.8+3.1

source:94-1851-1c/o^M14
 
Region Phenotype No. Totals
0 bz Wx 177  
  + wx 133 310
1 + Wx 71  
  bz wx 30 101

% recombination bz2-Wx1= 24.6+2.1

2) 2 point linkage data for f1-wx1 T1-9(4995)

Testcross: [F1 wx1 1-9(4995) x f1 wx1 N] x f1 wx1 N

source:94-1845-1c/o^M14
 
Region Phenotype No. Totals
0 f Wx 830  
  + wx 724 1554
1 + Wx 79  
  f wx 48 127

% recombination f1-Wx1= 7.6+0.6

Testcross: [f1 wx1 N x F1 wx1 1-9(4995)] x f1 wx1 N

source:94-1845-2c/o^M14
 
Region Phenotype No. Totals
0 f Wx 760  
  + wx 704 1464
1 + Wx 87  
  f wx 60 147

% recombination f1-Wx1= 9.1+0.7

Testcross: [F1 wx1 1-9(4995) x f1 wx1 N] x f1 wx1 N

source:94-1851-1c/o^M14
 
Region Phenotype No. Totals
0 f Wx 975  
  + wx 970 1945
1 + Wx 95  
  f wx 66 161

% recombination f1-Wx1= 7.6+0.6

Testcross: [f1 wx1 N x F1 wx1 1-9(4995)] x f1 wx1 N

source:94-1851-2c/o^M14
 
Region Phenotype No. Totals
0 f Wx 727  
  + wx 662 1389
1 + Wx 82  
  f wx 35 117

% recombination f1-Wx1= 7.8+0.7

B) The W23 source showed linkage of wx1 with bz2 & f1.

1) 2 point linkage data for bz2-wx1 T1-9(4995)

Testcross: bz2 wx1 N x [bz2 wx1 N x Bz2 wx1 1-9(4995)]

source:84H-2410-4^W23
 
Region Phenotype No. Totals
0 bz Wx 329  
  + wx 233 562
1 + Wx 108  
  bz wx 93 201

% recombination bz2-Wx1= 26.3+1.6

2) 2 point linkage data for f1-wx1 T1-9(4995)

Testcross: [f1 wx1 N x F1 wx1 1-9(4995)] x f1 wx1 N

source:84H-2410-4^W23
 
Region Phenotype No. Totals
0 f Wx 1111  
  + wx 975 2086
1 + Wx 85  
  f wx 52 137

% recombination f1-Wx1= 6.2+0.5

C) The new Sisco c/o source showed linkage of wx1 with bz2.

1) 2 point linkage data for bz2-wx1 T1-9(4995)

Testcross: [bz2 wx1 N x Bz2 wx1 1-9(4995)] x bz2 wx1 N

source:94-1841-9c/o^Sisco
 
Region Phenotype No. Totals
0 bz Wx 714  
  + wx 530 1244
1 + Wx 434  
  bz wx 247 681

% recombination bz2-Wx1= 35.4+1.1

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Table 3. wx1 T2-9c (2S.49; 9S.33)

A) TheF1 source showed linkage of wx1 with lg1gl2.

3 point linkage data for lg1 gl2-wx1 T2-9c

Testcross: [lg1 gl2 Wx1 N x Lg1 Gl2 wx1 T2-9c] x lg1 gl2 wx1 N

source:93-432-2 x 433 ^F1
 
Region Phenotype No. Totals
0 lg gl Wx 748  
  + + wx 1289 2037
1 + gl Wx 178  
  lg + wx 277 455
2 + + Wx 115  
  lg gl wx 94 209
1+2 lg + Wx 9  
  + gl wx 3 12

% recombination lg1-g12 =17.2+0.7

% recombination gl2-Wx1=8.1+0.5

% recombination lg1-Wx1=24.9+0.8

B) The W23 source showed linkage of wx1 with lg1gl2:

3 point linkage data for lg1 gl2-wx1 T2-9c

Testcross: [lg1 gl2 Wx1 N x Lg1 Gl2 wx1 T2-9c] x lg1 gl2 wx1 N

source:87-838 x 839 ^W23
 
Region Phenotype No. Totals
0 lg gl Wx 378  
  + + wx 745 1123
1 + gl Wx 105  
  lg + wx 159 264
2 + + Wx 58  
  lg gl wx 64 122
1+2 lg + Wx 4  
  + gl wx 4 8

% recombination lg1-g12 =17.9+1.0

% recombination gl2-Wx1=8.6+0.7

% recombination lg1-Wx1=25.9+1.1

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Table 4. wx1 T6-9(4505) (6L.13; 9ctr.)

A) The M14 sources showed linkage of wx1 with y1:

1) 2 point linkage data for y1-wx1 T6-9(4505)

Testcross: [Y1 wx1 T6-9(4505) x y1 Wx1 N] x y1 wx1 N

source:93W-1411-3^M14
 
Region Phenotype No. Totals
0ZZ y Wx 177  
  + wx 194 371
1 + Wx 13  
  y wx 6 19

% recombination y1-Wx1=4.9+1.1

source:94-1965-9c/o^M14
 
Region Phenotype No. Totals
0 y Wx 955  
  + wx 846 1801
1 + Wx 47  
  y wx 63 110

% recombination y1-Wx1=5.8+0.5

source:95-1034-4 fr. 94-1965-9c/o^M14
 
Region Phenotype No. Totals
0 y Wx 736  
  + wx 678 1414
1 + Wx 84  
  y wx 44 128

% recombination y1-Wx1=8.3+0.7

source:94-1965-10c/o^M14
 
Region Phenotype No. Totals
0 y Wx 675  
  + wx 571 1246
1 + Wx 31  
  y wx 33 64

% recombination y1-Wx1=4.9+0.6

source:94-1965-11c/o^M14
 
Region Phenotype No. Totals
0 y Wx 817  
  + wx 832 1649
1 + Wx 48  
  y wx 40 88

% recombination y1-Wx1=5.1+0.5

source:94-1965-12c/o^M14
 
Region Phenotype No. Totals
0 y Wx 684  
  + wx 640 1324
1 + Wx 38  
  y wx 32 70

% recombination y1-Wx1=5.0+0.6

B) The W23 sources showed linkage of wx1 with y1:

1) 2 point linkage data for y1-wx1 T6-9(4505)

Testcross: [Y1 Wx1 N x y1 wx1 T6-9(45045)] x y1 wx1 N

source: 87-910 x sib bulk 1^W23
 
Region Phenotype No. Totals
0 + Wx 237  
  y wx 209 446
1 y Wx 34  
  + wx 31 65

% recombination y1-Wx1=12.7+1.5

source:87-911-2 x sib^W23
 
Region Phenotype No. Totals
0 + Wx 593  
  y wx 523 1116
1 y Wx 136  
  + wx 134 270

% recombination y1-Wx1=19.5+1.1

Testcross: y1 wx1 N x [Y1 Wx1 N x y1 wx1 T6-9(45045)]

source:87-911-2 x sib^W23
 
Region Phenotype No. Totals
0 + Wx 641  
  y wx 566 1207
1 y Wx 47  
  + wx 60 107

% recombination y1-Wx1=8.1+0.8

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Table 5. wx1 T8-96921 (8L.85; 9L.15)

A) The Robertson Accession source showed linkage of wx1 with j1:

2 point linkage data for j1-wx1 T8-9(6921)

Testcross: [J1 wx1 T8-9(6921) x j1 Wx1 N] x j1 wx1 N

source:90-563-1^Rob.
 
Region Phenotype No. Totals
0 j Wx 39  
  + wx 51 90
1 + Wx 1  
  j wx 1 2

% recombination j1-Wx1=2.2+1.5
 
 


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