Three-point linkage data for Og*-0376 Wx1 T9-10b on 10S --Jackson, JD A new dominant yellow stripe stock maps to chromosome 10S near Og1. This new mutation was isolated from a ms2 stock of E. B. Patterson. Its phenotype is very similar to Og1. Crosses were done with the waxy1-marked translocations: T9-10b and T8-9(043-6) to genetically determine its chromosomal loction.

The results of a three-point linkage test for Wx1, T9-10b and Og*-0376 are presented in Table 1. The linkage test was set up as a modified backcross. Wx and wx kernels from the backcross ears were planted in the field and the resulting plants were scored for yellow stripes and for the presence of the translocation by pollen sterility. The following linkage relationship was established: Wx1 9.7 T9-10b 3.2 Og*-0376. Crosses are underway with Og1 to determine if Og*-0376 is an allele of Og1.

The results of a two-point linkage test for Wx1, T8-9(043-6) and Og*-0376 are presented in Table 2. The linkage test was also set up as a modified backcross. Wx and wx kernels from the backcross ears were planted in the field and the resulting plants were scored for yellow stripes. Results indicate no linkage of Og*-0376 with either chromosome 9 or chromosome 8.

Table 1. Three point linkage data for Og*-0376-Wx1-T9-10b.

Testcross: og* wx1 N x [Og*-0376 Wx1 N x og* wx1 T9-10b]
 
Region Phenotype No. Totals
0 Wx N str 44  
  wx T gr 38 82
1 Wx T gr 4  
  wx N str. 4 8
2 Wx N gr 2  
  wx T str 0 2
1+2 Wx T str 0  
  wx N gr 1 1

% recombination Wx1-T =9.7+2.9

% recombination T-Og*=3.2+1.4

% recombination Wx1-*Og=11.8+3.3

Table 2. Two point linkage data for Og*-0376-Wx1-T8-9(043-6).

Testcross: og* wx1 N x [Og*-0376 Wx1 N x og* wx1 T8-9(043-6)]
 
Region Phenotype No. Totals
0 Wx str 15  
  wx gr 28 43
1 Wx gr 32  
  wx str 13 45

% recombination Wx1*-Og= 51.1+5.3
 
 


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