Three-point linkage data for fl2 bm3 su1 on 4S --Stinard, PS The results of a three-point linkage test for fl2, bm3, and su1 on chromosome 4 are presented in Table 1. The linkage test was set up as a modified backcross as indicated in Table 1. Kernels from the backcross ears were planted in the field and the resulting plants were self-pollinated and scored for bm3, and the self-pollinated ears were scored for the presence of fl2 and su1. The following linkage relationship was established: fl2 - 5.0 - bm3 - 2.1 - su1. These data are consistent with the fl2 - su1 distance (8 cM) given on the most recent genetic map of chromosome 4, and clearly place bm3 distal to the TB-4Sa breakpoint.

Table 1. Three-point linkage data for fl2 - bm3 - su1.

Testcross: (fl2 Bm3 Su1 / Fl2 bm3 su1) X Fl2 bm3 Su1.
 
Reg. Phenotype No. Totals
       
0 fl2 + +  208  
  + bm3 su1 197 405
       
1 fl2 bm3 su1 8  
  + + +  14 22
       
2 fl2 + su1 3  
  + bm3 +  6 9
       
1+2 fl2 bm3 +  0  
  + + su1 0 0

% recombination fl2--bm3 = 5.0 +/- 1.0

% recombination bm3--su1 = 2.1 +/- 0.7

% recombination fl2--su1 = 7.1 +/- 1.2
 
 


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