BUFFALO, NEW YORK
Williamsville North High School
State University of New York
University of Western Ontario
Abnormal node development in na1/na1
--Cheng, W, Cheng, P-c, Walden, DB
The phenotype of na1/na1 is characterized
as dwarf plant. The dwarf character is associated with an abnormal development
in the stem. Instead of the well-defined nodes and internodes found in
the normal wild type stem, na1/na1 develops a stem lacking organized
nodal and internodal vasculature. Different from the parallel longitudinal
vascular bundles found in the internode of wild type, na1/na1 has
a poorly structured arrangement. In addition, the interconnecting vascular
bundles are not limited to the nodal region as found in the wild type,
but can be found at any level of the stem (arrows).
Figure 1 is a longitudinal section of
a na1/na1 stem showing the stem with the aforementioned features.
The plant was grown in 1999 summer nursery at the field station of University
of Western Ontario, London, Canada. The specimen was fixed in 1:3 acetic
acid/ EtOH, stained in diluted Schiff’s reagent (10%), washed in 0.1 M
metabisulphate solution (with 1 ml of 1 N HCl per liter), dehydrated in
EtOH and cleared in methyl salicylate. Note the vascular bundles extend
into the leaves but are not connected to a nodal structure (double-arrows).
Please Note: Notes submitted to the Maize Genetics Cooperation Newsletter may be cited only with consent of the authors.
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