COLUMBIA, MISSOURI
University of Missouri
URBANA, ILLINOIS
University of Illinois and USDA/ARS
Genetic mapping of zebra3 --Rugen, M., Stinard, PS, Cone, KC The recessive zebra3 (zb3) phenotype is apparent as a distinct light-green crossbanding on seedling and adult leaves of the maize plant. In an earlier study (MNL 73:23), we showed that previous reports that zb3 maps on 5L were incorrect. To determine the correct map location, zb3 was crossed by a series of TB stocks; the results indicated that zb3 is on the short arm of chromosome 1. For more specific localization, we used molecular segregation analysis. We generated a segregating population by crossing zb3 (Coop stock 519G) to a W23/L317 hybrid and then self-pollinating the F1 to generate an F2. F2 seed were planted and the phenotypes scored; 27 of 100 plants had the zb3 phenotype. DNA was isolated from the zb3 plants, digested with restriction enzymes, blotted and probed with markers from chromosome 1S. The following data were obtained:
 
probe
# chromosomes tested
# crossovers
recombination frequency (%)
tub1
54
0
0
asg31
54
3
5.6
umc157
54
2
3.7
asg45
54
8
14.8

We conclude that zb3 is tightly linked to tub1.
 
 


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