Additional linkage tests of non-waxy (Waxy1) reciprocal translocations involving chromosome 9 at the MGCSC --Jackson, JD, Stinard, P, Zimmerman, S Approximately one acre each year is devoted to the propagation of the large collection of A-A translocation stocks. In this collection is a series of Waxy1-linked translocations that are used for mapping unplaced mutants. Each translocation is maintained in separate M14 and W23 inbred backgrounds which are crossed together to produce vigorous F1's to fill seed requests. Over the years, pedigree and classification problems arose during the propagation of these stocks. We have been able to sort through the problem ones, and can now supply good sources proven by linkage tests to include the correct translocated chromosomes.

Previously we reported the linkage results for some of these stocks (MNL72:79-81; MNL73:86-88; MNL74:67). Below is a summary of additional translocation stocks we have completed testing.

Table 1. Wx1 T4-9b (4L.90; 9L.29)

A) The F1 source showed linkage of wx1 with c2.

2 point linkage data for c2-Wx1 T4-9b
Testcross: [C2 Wx1 T4-9b x c2 wx1 N] x c2 wx1 N

source: 87-998 x 996^F1
Region Phenotype No. Totals
0 + Wx 829  
  cl wx 1039 1868
1 cl Wx 100  
  + wx 287 387
% recombination c2-wx1= 17.2+0.8

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Table 2. Wx1 T6-9(4505) (6L.13; 9ctr.)

A) The F1 source showed linkage of wx1 with y1:

2 point linkage data for Y1-Wx1 T6-9(4505)
Testcross: [Y1 Wx1 T6-9(4505) x y1 wx1 N] x y1 wx1 N

source: 87-1027 x 1025 ^F1
Region Phenotype No. Totals
0 + Wx 1391  
  y wx 1468 2859
1 y Wx 204  
  + wx 187 391
% recombination y1-wx1= 12.0+0.6

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Table 3. Wx1 T7-9a (7L.63; 9S.07)

A) The F1 source showed linkage of wx1 with gl1:

2 point linkage data for gl1-Wx1 T7-9a
Testcross: [Gl1 Wx1 T7-9a x gl1 wx1 N] x gl1 wx1 N

source: 87-1038 x 1036^F1
Region Phenotype No. Totals
0 + Wx 955  
  gl wx 846 1801
1 gl Wx 186  
  + wx 173 359
% recombination gl1-wx1= 16.6±0.8

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Table 4. Wx1 T8-9d (8L.09; 9L.16)

A) The M14 source showed linkage of wx1 with v16:

2 point linkage data for v16-Wx1 T8-9d
Testcross: [V16 Wx1 T8-9d x v16 wx1 N] x v16 wx1 N

source: 888-999-1001 x same bulk #1^M14
Region Phenotype No. Totals
0 + Wx 263  
  v wx 221 484
1 v Wx 141  
  + wx 133 274
% recombination v16-wx1=36.1±1.7

B) The W23 source showed linkage of wx1 with v16:

2 point linkage data for v16-Wx1 T8-9d
Testcross: [V16 Wx1 T8-9d x v16 wx1 N] x v16 wx1 N

source: 82-157-2@^W23
Region Phenotype No. Totals
0 + Wx 575  
  v wx 521 1096
1 v Wx 239  
  + wx 189 428
% recombination v16-wx1=28.1±1.2

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Table 5. Wx1 T8-9(6673) (8L.35; 9S.31)

A) The M14 source showed linkage of wx1 with v16:

2 point linkage data for v16-Wx1 T8-9(6673)
Testcross: [V16 Wx1 T8-9(6673) x v16 wx1 N] x v16 wx1 N

source: 87-1715-1 x sib^M14
Region Phenotype No. Totals
0 + Wx 554  
  v wx 428 982
1 v Wx 139  
  + wx 182 321
% recombination v16-wx1=24.6±1.2

B) The W23 source showed linkage of wx1 with v16:

2 point linkage data for v16-Wx1 T8-9(6673)
Testcross: [V16 Wx1 T8-9(6673) x v16 wx1 N] x v16 wx1 N

source: 87-1716-1 x sib^W23
Region Phenotype No. Totals
0 + Wx 445  
  v wx 375 820
1 v Wx 208  
  + wx 186 394
% recombination v16-wx1=32.5±1.3
 
 


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