Additional linkage tests of waxy1 marked reciprocal translocations at the MGCSC --Jackson, JD, Stinard, P, Zimmerman, S In the collection of A-A translocation stocks maintained at MGCSC is a series of waxy1-linked translocations that are used for mapping unplaced mutants. Also new wx1-linked translocations are being introduced into this series and are in a conversion program to transfer each translocation to the inbred backgrounds M14 and W23. These inbreds are then crossed together to produce vigorous F1's to fill seed requests. Over the years, pedigree and classification problems arose during the propagation of these stocks. We have been able to sort through the problem ones, and can now supply good sources proven by linkage tests to include the correct translocated chromosomes. Additional pedigree information on bad sources is available should anyone want to check on samples supplied to them previously by the Stock Center.

Previously we reported the linkage results for some of these stocks (MNL72:81-82; MNL73:88-89; MNL74:67-69). Below is a summary of additional translocation stocks for which we have completed testing. Additional translocation stocks will be tested as time allows.

Table 1. wx1 T3-9c (3L.09; 9L.12)

A) The M14 source showed linkage of wx1 with vp1:

3 point linkage data for vp1-wx1 T3-9c
Modified backcross: [Vp1 wx1 N x [vp1 Wx1 N x Vp1 wx1 T3-9c]]@

source: 93-481-1 ^M14
Region Phenotype No. Totals
0 vp N Wx 129  
  + T wx 108 237
1 + N Wx 18  
  vp T wx 9 27
2 + T Wx 4  
  vp N wx 1 5
1+2 vp T Wx 2  
  + N wx 0 2
% recombination vp1-wx1 =13.3+2.1
% recombination vp1-T=10.7+1.9
% recombination T-wx1=2.6+1.

B) The W23 source showed linkage of wx1 with vp1:

3 point linkage data for vp1-wx1 T3-9c
Modified backcross: [Vp1 wx1 N x [vp1 Wx1 N x Vp1 wx1 T3-9c]]@

source: 94-1893-1 ^W23
Region Phenotype No. Totals
0 vp N Wx 153  
  + T wx 170 323
1 + N Wx 17  
  vp T wx 10 27
2 + T Wx 1  
  vp N wx 1 2
1+2 vp T Wx 2  
  + N wx 2 4
% recombination vp1-wx1 =10.4+1.6
% recombination vp1-T=8.7+1.5
% recombination T-wx1=1.7+0.7

C) Combined data for M14 & W23 sources.

3 point linkage data for vp1-wx1 T3-9c
Modified backcross: [Vp1 wx1 N x [vp1 Wx1 N x Vp1 wx1 T3-9c]]@

combined M14 & W23 data
Region Phenotype No. Totals
0 vp N Wx 278  
  + T wx 282 560
1 + N Wx 35  
  vp T wx 19 54
2 + T Wx 5  
  vp N wx 2 7
1+2 vp T Wx 4  
  + N wx 2 6
% recombination vp1-wx1 =11.6+1.3
% recombination vp1-T=9.6+1.1
% recombination T-wx1=2.1+0.4

The following linkage relationship was established: vp1 9.6 - T 2.1 - wx1. These data are consistent with the % recombination vp1-wx1 = 11.06+0.62 in Robertson, D.S. 1955. Genetics 40:745-760. These could also be consistent with the breakpoints determined by Dr. C. R. Burnham and students, University of Minnesota.

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Table 2. wx1 T4-9g (4S.27; 9L.27)

A) The F1 source showed linkage of wx1 with su1.

2 point linkage data for su1-wx1 T4-9g
Testcross: [Su1 wx1 T4-9g x su1 Wx1 N] x su1 wx1 N

source: 93-444-2 x 445 F1 of ^M14 x ^W23
Region Phenotype No. Totals
0 + wx 749  
  su Wx 741 1490
1 + Wx 241  
  su wx 23 264
% recombination su1-wx1=15.1+.09

B) The M14 source showed linkage of wx1 with su1:

2 point linkage data for su1-wx1 T4-9g
Testcross: [Su1 wx1 T4-9g x su1 Wx1 N] x su1 wx1 N

source: 93-482-1 x SIB^M14
Region Phenotype No. Totals
0 + wx 810  
  su Wx 818 1625
1 + Wx 247  
  su wx 18 265
% recombination su1-wx1=14.0+0.8

C) The W23 source showed linkage of wx1 with su1:

2 point linkage data for su1-wx1 T4-9g
Testcross: [Su1 wx1 T4-9g x su1 Wx1 N] x su1 wx1 N

source: 92-405-1 x SIB^W23
Region Phenotype No. Totals
0 + wx 1007  
  su Wx 998 2005
1 + Wx 284  
  su wx 31 315
% recombination su1-wx1=13.6±0.7

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Table 3. wx1 T4-9e (4S.53; 9L.26)

A) The F1 source showed linkage of wx1 with su1.

2 point linkage data for su1-wx1 T4-9e
Testcross: [Su1 wx1 T4-9e x su1 Wx1 N] x su1 wx1 N

source: 93W-1389-1 x 1390-9 F1 of ^M14 x ^W23
Region Phenotype No. Totals
0 + wx 478  
  su Wx 604 1082
1 + Wx 70  
  su wx 19 89
% recombination su1-wx1=7.6±0.8

B) The M14 source showed linkage of wx1 with su1:

2 point linkage data for su1-wx1 T4-9e
Testcross: [Su1 wx1 T4-9e x su1 Wx1 N] x su1 wx1 N

source: 92H-470-1 x SIB^M14
Region Phenotype No. Totals
0 + wx 934  
  su Wx 1052 1986
1 + Wx 39  
  su wx 10 49
% recombination su1-wx1=2.4±0.3

C) The W23 source showed linkage of wx1 with su1:

2 point linkage data for su1-wx1 T4-9e
Testcross: [Su1 wx1 T4-9e x su1 Wx1 N] x su1 wx1 N

source: 93W-1390-9@^W23
Region Phenotype No. Totals
0 + wx 1018  
  su Wx 1033 2051
1 + Wx 72  
  su wx 37 109
% recombination su1-wx1=5.0±0.5

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Table 4. wx1 T4-9(5657) ( (4L.33; 9S.25)

A) The F1 source showed linkage of wx1 with gl4:

2 point linkage data for gl4-wx1 T4-9(5657)
Testcross: [Gl4 wx1 T4-9(5657) x gl4 Wx1 N] x gl4 wx1 N

source: 87-870 x 872^F1
Region Phenotype No. Totals
0 gl Wx 240  
  + wx 225 465
1 + Wx 12  
  gl wx 5 17
% recombination gl4-wx1= 3.5±0.8

B) The W23 source showed linkage of wx1 with gl4:

2 point linkage data for gl4-wx1 T4-9(5657)
Testcross: [Gl4 wx1 T4-9(5657) x gl4 Wx1 N] x gl4 wx1 N

source: 92H-471-7^W23
Region Phenotype No. Totals
0 gl Wx 92  
  + wx 83 175
1 + Wx 10  
  gl wx 3 13
% recombination gl4-wx1=6.9±1.9

C) The M14 source was not tested.

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Table 5. wx1 T5-9(4817) (5L.06; 9S.07)

A) The F1 source showed linkage of wx1 with a2:

2 point linkage data for a2-wx1 T5-9(4817)
Testcross: [A2 wx1 T5-9(4817) x a2 Wx1 N] x a2 wx1 N

source: 933-450-1 x 451^F1
Region Phenotype No. Totals
0 a Wx 494  
  + wx 421 915
1 + Wx 63  
  a wx 27 90
% recombination a2-wx1=9.0±0.9

B) The M14 source showed linkage of wx1 with a2:

2 point linkage data for a2-wx1 T5-9(4817)
Testcross: [A2 wx1 T5-9(4817) x a2 Wx1 N] x a2 wx1 N

source: 87-884 x SIB ^M14
Region Phenotype No. Totals
0 a Wx 1006  
  + wx 959 1965
1 + Wx 133  
  a wx 49 182
% recombination a2-wx1=8.5±0.6

C) The W23 source showed linkage of wx1 with a2.

2 point linkage data for a2-wx1 T5-9(4817)
Testcross: [A2 wx1 T5-9(4817) x a2 Wx1 N] x a2 wx1 N

source: 92-412-1 x SIB ^W23
Region Phenotype No. Totals
0 a Wx 802  
  + wx 781 1583
1 + Wx 104  
  a wx 47 151
% recombination a2-wx1=8.7±0.7

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Table 6. wx1 T5-9d (5L.14; 9L.10)

A) The F1 source showed linkage of wx1 with gl8:

2 point linkage data for gl8-wx1 T5-9d
Testcross: [Gl8 wx1 T5-9d x gl8 Wx1 N] x gl8 wx1 N

source:93-452-3 x 453 ^F1
Region Phenotype No. Totals
0 gl Wx 598  
  + wx 565 1163
1 + Wx 193  
  gl wx 111 304
% recombination gl8-wx1= 20.7±1.1

B) The M14 source showed linkage of wx1 with gl8:

2 point linkage data for gl8-wx1 T5-9d
Testcross: [Gl8 wx1 T5-9d x gl8 Wx1 N] x gl8 wx1 N

source: 93-483-1 x SIB ^M14
Region Phenotype No. Totals
0 gl Wx 864  
  + wx 819 1683
1 + Wx 220  
  gl wx 147 367
% recombination gl8-wx1=17.9±08

C) The W23 source showed linkage of wx1 with gl8.

2 point linkage data for gl8-wx1 T5-9d
Testcross: [Gl8 wx1 T5-9d x gl8 Wx1 N] x gl8 wx1 N

source: 93W-1414-3@^W23
Region Phenotype No. Totals
0 gl Wx 430  
  + wx 419 849
1 + Wx 159  
  gl wx 91 250
% recombination gl8-wx1=22.7±1.3

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Table 7. wx1 T8-9d (8L.09; 9L.16)

A) The F1 source showed linkage of wx1 with v16:

2 point linkage data for v16-wx1 T8-9d
Testcross: [V16 wx1 T8-9d x v16 Wx1 N] x v16 wx1 N

source: 93-470-1 x 471^F1
Region Phenotype No. Totals
0 v Wx 356  
  + wx 336 692
1 + Wx 200  
  v wx 109 309
% recombination v16-wx1=30.9±1.5

B) The M14 source showed linkage of wx1 with v16:

2 point linkage data for v16-wx1 T8-9d
Testcross: [V16 wx1 T8-9d x v16 Wx1 N] x v16 wx1 N

source: 93-490-1@^M14
Region Phenotype No. Totals
0 v Wx 600  
  + wx 492 1092
1 + Wx 158  
  v wx 81 239
% recombination v16-wx1=18.0±1.1

C) The W23 source showed linkage of wx1 with v16:

2 point linkage data for v16-wx1 T8-9d
Testcross: [V16 wx1 T8-9d x v16 Wx1 N] x v16 wx1 N

source: 92H-483-1 x SIB^W23
Region Phenotype No. Totals
0 v Wx 451  
  + wx 410 861
1 + Wx 146  
  v wx 95 241
% recombination v16-wx1=21.9±1.2

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Table 8. wx1 T8-9(6673) (8L.35; 9S.31)

A) The F1 source showed linkage of wx1 with v16:

2 point linkage data for v16-wx1 T8-9(6673)
Testcross: [V16 wx1 T8-9(6673) x v16 Wx1 N] x v16 wx1 N

source: 93-472-1 x 473^F1
Region Phenotype No. Totals
0 v Wx 643  
  + wx 542 1185
1 + Wx 141  
  v wx 111 252
% recombination v16-wx1=17.5±1.0

B) The M14 source showed linkage of wx1 with v16:

2 point linkage data for v16-wx1 T8-9(6673)
Testcross: [V16 wx1 T8-9(6673) x v16 Wx1 N] x v16 wx1 N

source: 93-433-1 x SIB^M14
Region Phenotype No. Totals
0 v Wx 403  
  + wx 376 779
1 + Wx 71  
  v wx 64 135
% recombination v16-wx1=14.8±1.2

C) The W23 source showed linkage of wx1 with v16:

2 point linkage data for v16-wx1 T8-9(6673)
Testcross: [V16 wx1 T8-9(6673) x v16 Wx1 N] x v16 wx1 N

source: 93-491-1 x SIB^W23
Region Phenotype No. Totals
0 v Wx 616  
  + wx 592 1208
1 + Wx 172  
  v wx 123 295
% recombination v16-wx1=19.6±1.0

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Table 9. wx1 T9-10(8630) (9S.28; 10L.37)

All wx1 marked sources of T9-10(8630) showed no linkage with either r1 or g1. New crossovers were obtained for both the M14 & W23 conversions.

A) The new M14 crossover sources showed linkage of wx1 with r1.

2 point linkage data for r1-wx1 T9-10(8630)
Testcross: [r1 wx1 T9-10(8630) x R1 Wx1 N] x r1 wx1 N

source: 99-1463-3; from: 98-1139-2c/o^M14
Region Phenotype No. Totals
0 + Wx 766  
  r wx 648 1414
1 r Wx 115  
  + wx 195 310
% recombination r1-wx1=18.0±0.9

source: 99-1463-5; from: 98-1139-2c/o^M14
Region Phenotype No. Totals
0 + Wx 779  
  r wx 571 1350
1 r Wx 90  
  + wx 159 249
% recombination r1-wx1=15.6±0.9

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B) The new M14 crossover sources showed linkage of wx1 with g1.

2 point linkage data for g1-wx1 T9-10(8630)
Testcross: [G1 wx1 T9-10(8630) x g1 Wx1 N ] x g1 wx1 N

source: 99-1463-2 from: 98-1139-2c/o^M14
Region Phenotype No. Totals
0 g Wx 1458  
  + wx 1254 2712
1 + Wx 76  
  g wx 70 146
% recombination g1-wx1=5.1±0.4

source: 99-1463-5 from: 98-1139-2c/o^M14
Region Phenotype No. Totals
0 g Wx 487  
  + wx 463 950
1 + Wx 17  
  g wx 11 28
% recombination g1-wx1=2.9±0.5

source: 99-1463-7 from: 98-1139-2c/o^M14
Region Phenotype No. Totals
0 g Wx 195  
  + wx 207 402
1 + Wx 5  
  g wx 5 10
% recombination g1-wx1=2.4±0.8

source: 99-1464-3 from: 98-1139-2c/o^M14
Region Phenotype No. Totals
0 g Wx 613  
  + wx 586 1199
1 + Wx 26  
  g wx 22 48
% recombination g1-wx1=3.8±0.5

source: 99-1464-6 from: 98-1139-2c/o^M14
Region Phenotype No. Totals
0 g Wx 215  
  + wx 221 436
1 + Wx 8  
  g wx 7 15
% recombination g1-wx1=3.3±0.8

source: 99-1464-8 from: 98-1139-2c/o^M14
Region Phenotype No. Totals
0 g Wx 696  
  + wx 649 1345
1 + Wx 22  
  g wx 13 35
% recombination g1-wx1=2.5±0.4

C) The new W23 crossover sources showed linkage of wx1 with g1.

2 point linkage data for g1-wx1 T9-10(8630)
Testcross: [G1 wx1 T9-10(8630) x g1 Wx1 N ] x g1 wx1 N

source: 99-1465-11 from: 98-1159-11c/o^W23
Region Phenotype No. Totals
0 g Wx 513  
  + wx 526 1040
1 + Wx 8  
  g wx 11 19
% recombination g1-wx1=1.8±0.4

source: 99-1466-1 from: 98-1159-11c/o^W23
Region Phenotype No. Totals
0 g Wx 494  
  + wx 457 951
1 + Wx 10  
  g wx 17 27
% recombination g1-wx1=2.8±0.5

source: 99-1468-2 from: 98-1159-11c/o^W23
Region Phenotype No. Totals
0 g Wx 527  
  + wx 463 990
1 + Wx 11  
  g wx 22 33
% recombination g1-wx1=3.2±0.6

source: 99-1468-8 from: 98-1159-11c/o^W23
Region Phenotype No. Totals
0 g Wx 246  
  + wx 247 493
1 + Wx 8  
  g wx 6 14
% recombination g1-wx1=2.8±0.7

source: 99-1468-11 from: 98-1159-11c/o^W23
Region Phenotype No. Totals
0 g Wx 335  
  + wx 661 645
1 + Wx 8  
  g wx 8 16
% recombination g1-wx1=2.4±0.6
 
 


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