two-point linkage data for inr1 and du1 on 10L
Inr1 is a dominant R1 allele-specific
aleurone color inhibitor that can be scored as pale or colorless aleurone
in the presence of certain susceptible R1 (R1-S) alleles.
The recessive inr1 allele provides full purple aleurone color in
the presence of R1-S. Previous data (Stinard, P. 1999. MNL 73:89-90)
had shown that inr1 is located on the long arm of chromosome 10,
distal to the TB-10L19 breakpoint (10L.00), but proximal to g1.
In order to further refine the map location of inr1, a three-point
linkage test involving the 10L markers inr1, du1, and g1
was set up as indicated in Table 1. All stocks used in the crosses were
homozygous for wx1 in order to enhance the expression of du1.
Kernels from these crosses will be planted at a later date and the resulting
plants scored for g1 in order to provide three-point linkage data.
Preliminary two-point data involving only inr1 and du1 are
presented in Table 1. These data indicate that inr1 and du1
are very tightly linked, with only an approximately 0.2 centimorgan distance
between them. These data cannot be used to determine the global order of
inr1 and du1 on chromosome 10. That determination will have
to be made after the g1 data are collected.
Table 1. Two-point linkage data for
inr1 and du1.
Testcross: (Du1 Inr1 G1 R1-S wx1
X du1 inr1 g1 R1-S wx1) X du1 inr1 g1 R1-S wx1.
% recombination inr1--du1
= 0.17 +/- 0.04
Please Note: Notes submitted to the Maize Genetics Cooperation Newsletter may be cited only with consent of the authors.
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