USDA/ARS/MWA - Soybean/Maize Germplasm, Pathology & Genetics Research Unit
University of Illinois at Urbana/Champaign - Department of Crop Sciences
1102 South Goodwin Avenue
Urbana, IL 61801-4798
(217) 333-6064 [fax]
1,674 seed samples have been supplied in response to 252 requests, for 2000. Of these, a total of 85 requests were received from 20 foreign countries. Approximately 90% of our requests were received by electronic mail or through our order form on the World Wide Web.
We have added more stocks to our ‘Phenotype Only’ category of stocks. These are stocks that have been donated to the COOP over the years, and have been classified according to their mutant phenotype only. For the most part, these stocks have not as yet been allele tested, nor has their gene been located to a chromosome arm. While we expect that most of these will represent new alleles of known loci, some will represent unique, as yet undescribed loci. Over the past few years, some mutants in this class have been mapped and/or allele tested and where appropriate, the now characterized mutant stock was added to our main catalog. We are now listing all of these mutants to give cooperators that are interested in specific traits, easier access to these mutants. These now include many mutants from Gerry Neuffer’s collection.
Approximately 6.2 acres of nursery were grown this summer at the Crop Sciences Research & Education Center located at the University of Illinois. Warm soil temperatures allowed for excellent emergence followed by optimal summer growing conditions. With additional water supplied by irrigation, we obtained good increases of most stocks grown this year
Special plantings were made of several categories of stocks:
2. Plantings were also made from donated stocks from the collections of Don Auger (translocated Ac lines), Ed Coe (various genetic stocks), James Brewbaker (aphid resistant lines), Greg Doyle (inversions), Ina Golubovskaya (dsy2 and Mei1), Al Kriz (globulin variants), Mario Motto (opaque and glossy alleles), Oliver Nelson (bronze1 alleles and other mutants in his collection), Virginia Walbot (transposon-induced aleurone color mutant alleles), David Weber (Trisomic 8), and others. We expect to receive additional accessions of stocks from maize geneticists within the upcoming year and again request Cooperators to send us their stocks to insure their existence for future researchers.
3. We conducted allelism tests of several categories of mutants with similar phenotype or chromosome location. We found additional alleles of sugary3, sugary4, yellow endosperm8, ramosa1, ramosa2, iojap1, green stripe1 and zebra necrotic2. In this manner, we hope to move stocks from our vast collection of unplaced uncharacterized mutants and integrate them into the main collection.
4. We conducted linkage tests of several mutants that had been placed to chromosome arm using B-A translocations or waxy-marked A-A translocations. More precise locations were determined for inhibitor of r1 and inhibitor of r2.
5. Two acres were devoted to the propagation of the large collection of cytological variants, including A-A translocation stocks and inversions. In this collection is a series of waxy1-marked translocations that are used for mapping unplaced mutants. Over the years, pedigree and classification problems arose during the propagation of these stocks. We were able to sort through the problem ones, and we can now supply good sources proven by linkage tests to include the correct translocated chromosomes. Many additional translocation stocks were tested this last year. Results of these tests are reported in this issue of the Maize Genetics Cooperation Newsletter.
6. Stocks produced from the NSF project "Maize Gene Discovery, Sequencing and Phenotypic Analysis" (see: http://zmdb.iastate.edu/) were grown this summer. Approximately 60% of these represented plants that originally had to be outcrossed, and needed to be selfed to analyze for mutant segregation. The remaining 40% were seed increases that were planted from those families that originally yielded poorly. These increases help to maintain adequate seed stock to fill future requests.
Philip continued his work on characterizing modifiers and inhibitors of certain R1 alleles, and the inheritance and expression of the duplicate factor pairs brn1 brn2 and su3 su4.
Janet continues with linkage testing and propagation of translocation stocks. She has finished sorting stocks into the ‘Phenotype Only’ category of stocks. Over the past year some mutants in this class have been allele tested and where appropriate, the now characterized mutant stock was added to our main catalog. Along with Shane she has sorted through the Neuffer collection and assigned each stock a drawer# and propagation status. All have been entered in a new database Shane set up to make retrieval of stocks easier.
The NSF project "Maize Gene Discovery,
Sequencing and Phenotypic Analysis" generated 9419 stocks that were sent
to the Stock Center. All of these stocks were then screened for ear and
kernel mutants, samples from each family were sent to UC Berkeley for plant
seedling screening and remaining seed was placed into cold storage until
requested. Results from these screenings can be found at the ZmDB: Phenotype
Database (http://zmfmdb.zool.iastate.edu/). Shane will work on the material
generated this past summer, that is now arriving here. He will also be
increasing stocks as necessary to maintain seed supply for requests and
planting many of these stocks for the observation of adult plant traits.
Our plan is to make this observation field available for maize genetics
cooperators to visit and search for mutants that they are interested in.
Details will be announced later.
|Janet Day Jackson
Senior Research Specialist
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