Eleven multiplasmic lines of maize were used as experimental materials, i.e. (Fli) Mo17 [original Mo17 line, (Fli) was shown as flint cytoplasm], (su1)Mo17, (sh2)Mo17, (bt1)Mo17, (Pop)Mo17, (wx)Mo17, (Teo)Mo17, (cms-T)Mo17, (cms-S)Mo17, (cms-C)Mo17, (cms-21A)Mo17. (Fli)Mo17 was used as a control (CK) to carry out the analyses. The common degree of nuclear genes was 99.95% in 10 of the multiplasmic lines.
Isozymes were determined as described by Zeng, M (Determinations of the biochemistry for CMS and genetics for their restoration in maize. Science in China, 25(3):283~296, 1987). The electrophoreses were conducted analyzing the POD and EST of kernel at the 9th day after pollination, embryo at the 15th, 25th, and 34th day after pollination, endosperm at the 15th, 25th, and 34th day after pollination, and young shoot of mature seed, unfolded leaf at the 6-leaf, 8-leaf, and 14-leaf stage, emergent tassel stage, pollination stage, 15th day after pollination, and tassel at emergent tassel, and pollen at pollination stage, respectively.
Experimental Results. The results obtained indicate there were 20 POD bands, and 18 EST bands in total. Comparing the 11 multiplasmic lines, the zymograms for POD electrophoresis of the different tissues at different stages varied clearly, formed the same bands and specific bands, that of embryo at the 34th day after pollination and pollen at pollination was uniform. The difference of zymogram for EST electrophoresis of the 11 multiplasmic lines mainly appeared in the tissues of the kernel at the 9th day, the embryo at the 25th day and 34th day, the endosperm at the 34th day after pollination, and the tassel at the emergent tassel and the pollen at the pollination stage, formed the same bands and specific bands also. But EST of the other tissues (that of the embryo at the 15th day, the endosperm at the 25th day and the endosperm at the 25th day after pollination, and the young shoot, the leaf at 6th leaf, at 8th leaf, at 14th leaf, at emergent tassel, at pollination, at the 15th day after pollination stage) was all uniform.
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