Study on male-sterility in Zea mays. XIII. RFLP analysis of mtDNA of
YII-1 type maize male-sterile cytoplasm
--Qin, T, Xu, M, Dun, D, Chen, R, Bian, Y
One set of male-sterile lines with the same nuclear backgrounds in maize,
Cms-Tu8112, Cms-Su8112, Cms-Cu8112, Cms-YII-1
U8112, were used in our experiments. MtDNA was prepared according
to Kemble, RJ. Hybridization has been described by Sambrook, J. Long-length
agarose gel electrophoresis can bring out high resolution for EcoRI,
BamHI and PstI digests of mtDNA of corn T, S, C groups and
new YII-1 type male-sterile cytoplasms. Hybridization of 5 mtDNA
specific probes to restricted mtDNA showed that many different hybridized
bands existed among T, S and C groups, The YII-1 type was different
from the T and S group, but there was little difference to the C group.
We would apply the technique of sequence analysis to the division of male-sterile
line YII-1 type.
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