Additional linkage tests of waxy1 marked reciprocal translocations at the MGCSC --Jackson, JD, Stinard, P, Zimmerman, S In the collection of A-A translocation stocks maintained at MGCSC is a series of waxy1-linked translocations that are used for mapping unplaced mutants. Also, new wx1-linked translocations are being introduced into this series and are in a conversion program to convert each translocation to the inbred backgrounds M14 and W23. These inbred conversions are then crossed together to produce vigorous F1's to fill seed requests. Over the years, pedigree and classification problems arose during the propagation of these stocks. We have been able to sort through the problem ones, and can now supply good sources proven by linkage tests to include the correct translocated chromosomes. Additional pedigree information on bad sources is available should anyone want to check on sources supplied to them previously by the Stock Center.

Previously we reported the linkage results for some of these stocks (MNL72:81-82; MNL73:88-89; MNL74:67-69; MNL75:68-71). Below is a summary of additional translocation stocks we have completed testing. Additional translocation stocks will be tested as time allows.

Table 1. wx1 T1-9(8389) (1L.74; 9L.13)

A) The F1 source showed linkage of wx1 with bm2:

Three point linkage data for bm2-T1-9(8389) wx1
Modified backcross: bm2 N wx1 x [bm2 N Wx1 x Bm2 T1-9(8389) wx1]

source: 93-930-3^F1
Region Phenotype No. Totals
0 + T wx 95  
  bm N Wx 82 177
1 + N Wx 36  
  bm T wx 53 89
2 + T Wx 14  
  bm N wx 4 18
1+2 + N wx 10  
  bm T Wx 9 19

% recombination bm2-T =35.6+2.8
% recombination T-wx1 =12.2+1.9
% recombination bm2-wx1 = 47.9+2.9

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Table 2. wx1 T2-9d (2L.83; 9L.27)

A) The M14 source showed linkage of wx1 with ch1:

Three point linkage data for Ch1-T2-9d wx1
Modified backcross: ch1 N wx1 x [Ch1 N Wx1 x ch1 T2-9d wx1 ]

source: 92-396-1^M14
Region Phenotype No. Totals
0 + T wx 40  
  Ch N Wx 63 103
1 + N Wx 36  
  Ch T wx 28 64
2 + T Wx 3  
  Ch N wx 4 7
1+2 + N wx 1  
  Ch T Wx 3 4

% recombination ch1-T =38.3+3.6
% recombination T-wx1=6.2+1.8
% recombination ch1-wx1=44.4+3.7

B) The W23 source showed linkage of wx1 with ch1:

Three point linkage data for Ch1-T2-9d wx1
Modified backcross: ch1 N wx1 x [Ch1 N Wx1 x ch1 T2-9d wx1 ]

source: 92-395-1^W23
Region Phenotype No. Totals
0 + T wx 94  
  Ch N Wx 92 186
1 + N Wx 27  
  Ch T wx 32 59
2 + T Wx 0  
  Ch N wx 0 0
1+2 + N wx 1  
  Ch T Wx 1 2
% recombination ch1-T =24.7+2.7
% recombination T-wx1=0.8+0.6
% recombination ch1-wx1=25.5+2.8

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Table 3. wx1 T3-9(8447) (3S.44; 9L.14)

A) The F1 source showed linkage of wx1 with gl6.

Two point linkage data for gl6-wx1 T3-9(8447)
Testcross: [Gl6 wx1 T3-9(8447) x gl6 Wx1 N] x gl6 wx1 N

source:93-438-1 x 439^F1
Region Phenotype No. Totals
0 gl Wx 503  
  + wx 452 955
1 + Wx 60  
  gl wx 53 113
% recombination gl6-wx1=10.6+0.9

B) The M14 source showed linkage of wx1 with gl6.

Two point linkage data for gl6-wx1 T3-9(8447)
Testcross: [Gl6 wx1 T3-9(8447) x gl6 Wx1 N] x gl6 wx1 N

source: 92-399-1@^M14
Region Phenotype No. Totals
0 gl Wx 437  
  + wx 448 885
1 + Wx 28  
  gl wx 30 58
% recombination gl6-wx1=6.2+0.8

C) The W23 source showed linkage of wx1 with gl6:

2 point linkage data for gl6-wx1 T3-9(8447)
Testcross: [Gl6 wx1 T3-9(8447) x gl6 Wx1 N] x gl6 wx1 N

source: 92-397-1 x SIB^W23
Region Phenotype No. Totals
0 gl Wx 1288  
  + wx 1298 2586
1 + Wx 111  
  gl wx 105 216
% recombination gl6-wx1=7.7+0.5

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Table 4. wx1 T4-9b (4L.90; 9L.29)

A) The F1 source showed linkage of wx1 with c2:

Two point linkage data for c2-wx1 T4-9b
Testcross: [C2 wx1 T4-9b x c2 Wx1 N] x c2 wx1 N

source: 93W-11392-1 x 1391-1^F1
Region Phenotype No. Totals
0 + wx 739  
  c2 Wx 929 1668
1 + Wx 82  
  c2 wx 89 171
% recombination c2-wx1=9.3+0.7

B) The M14 source showed linkage of wx1 with c2:

Two point linkage data for c2-wx1 T4-9b
Testcross: [C2 wx1 T4-9b x c2 Wx1 N] x c2 wx1 N

source: 92-410-1 x SIB^M14
Region Phenotype No. Totals
0 + wx 1801  
  c2 Wx 2077 3878
1 + Wx 295  
  c2 wx 206 501
% recombination c2-wx1=11.4+0.5

C) The W23 source showed linkage of wx1 with c2:

Two point linkage data for c2-wx1 T4-9b
Testcross: [C2 wx1 T4-9b x c2 Wx1 N] x c2 wx1 N

source: 92H-472-1 x SIB
Region Phenotype No. Totals
0 + wx 547  
  c2 Wx 568 1115
1 + Wx 59  
  c2 wx 65 124
% recombination c2-wx1=10.0+0.9

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Table 5. wx1 T6-9e (6L.18; 9L.24) (Source from D. Robertson)

A) The original source converted to M14 showed linkage of wx1 with y1:

Two point linkage data for y1-wx1 T6-9e
Testcross: [y1 wx1 T6-9e x Y1 Wx1 N] x y1 wx1 N

source: 93W-1487-4^M14
Region Phenotype No. Totals
0 y wx 1583  
  + Wx 1598 3181
1 + wx 190  
  y Wx 180 370
% recombination y1-wx1=10.4+0.5

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The following is a correction on data previously reported in MNL74:67-69.

Table 6. wx1 T2-9c (2S.49; 9S.33)

A) The F1 source showed linkage of wx1 with lg1gl2.

Three point linkage data for lg1 gl2-wx1 T2-9c
Testcross: [lg1 gl2 Wx1 N x Lg1 Gl2 wx1 T2-9c] x lg1 gl2 wx1 N

source: 93-432-2 x 433 ^F1
Region Phenotype No. Totals
0 lg gl Wx 748  
  + + wx 1289 2037
1 + gl Wx 178  
  lg + wx 277 455
2 + + Wx 115  
  lg gl wx 94 209
1+2 lg + Wx 9  
  + gl wx 3 12
% recombination lg1-g12 =17.2+0.7
% recombination gl2-wx1=8.1+0.5
% recombination lg1-wx1= 25.4+0.8 (originally reported to be 24.9+0.8)

B) The W23 source showed linkage of wx1 with lg1gl2:

Three point linkage data for lg1 gl2-wx1 T2-9c
Testcross: [lg1 gl2 Wx1 N x Lg1 Gl2 wx1 T2-9c] x lg1 gl2 wx1 N

source: 87-838 x 839 ^W23
Region Phenotype No. Totals
0 lg gl Wx 378  
  + + wx 745 1123
1 + gl Wx 105  
  lg + wx 159 264
2 + + Wx 58  
  lg gl wx 64 122
1+2 lg + Wx 4  
  + gl wx 4 8
% recombination lg1-g12 =17.9+1.0
% recombination gl2-wx1=8.6+0.7
% recombination lg1-wx1= 26.5+1.1 (originally reported to be 25.9+1.1 )
 
 


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